乙型肝炎病毒前C区变异患者血清Ⅳ型胶原/层粘连蛋白和一氧化氮的分析

目的：分析乙型肝炎病毒（ＨＢＶ）前Ｃ区基因变异在肝纤维化形成中的作用，探讨肝纤维化与一氧化氮的关系。方法：利用错配ＰＣＲ限制片段多态性分析，检测ＨＢＶ前Ｃ区１８９６位点突变，并检测慢性乙肝患者血清Ⅳ型胶原／层粘连蛋白（ＣⅣ／ＬＮ）和ＮＯ。结果：Ａ组３１例前Ｃ区变异或变异优势患者ＣⅣ（１２７．３５±２４．３３）μｇ／Ｌ，ＬＮ（１６９．１０±２８．２３）μｇ／Ｌ明显高于Ｂ组３０例无前Ｃ区基因变异患者ＣⅣ（８６．８４±１２．６６）μｇ／Ｌ，ＬＮ（９４．２０±１５．２９）μｇ／Ｌ（Ｐ＜０．００１），且Ａ组ＮＯ－２／ＮＯ－３（４８．５５±４．０３）μｍｏｌ／Ｌ明显高于Ｂ组ＮＯ－２／ＮＯ－３（３６．６０±４．０２）μｍｏｌ／Ｌ（Ｐ＜０．００１）。结论：ＨＢＶ前Ｃ区变异与肝纤维化、肝硬化发生发展有关系，且血清ＮＯ随ＣⅣ和ＬＮ增高而增高。

ANALYSINGE SERUM COLLAGEN /LAMININ AND NITRIC OXIDE IN PATIENTS WITH HBV PRE-C MUTATION

Objective:To analysis the effects of HBV pre-C mutation in hepatic fibrosis and discuss the relationship of hepatic fibrosis and nitric oxide.Methods:HBV pre-C nt 1896 mutation was tested by mis-pair PCR restriction fragment length polymorphism.The levels of serum collagen /laminin and NO-2/NO-3 were tested in patients with chronic hepatitis B infection.Results:Collagen (127.3524.33) g/L,laminin (169.1028.23) g/L in 31 patients of group A with whole mutation and superiority mutation were obviously higher than that of collagen (86.8412.66) g/L/laminin (94.2015.27) g/L in 30 patients of group B with no mutation,and the NO-2/NO-3 (48.554.03) mol/L in group A was also Obviously higher than the NO-2/NO-3(36.604.02) mol/L in group B.Conclusion:HBV pre-C mutation was related to hepatic fibrosis and cirrhosis.and the level of serum nitric oxide was increased with that of collagen /laminin.;