沈阳地区76例非何杰金淋巴瘤的分类及其免疫表型病理分析

目的：探讨我国沈阳地区非何杰金淋巴瘤（NHL）的病理特点及免疫组化表型分析。方法：对76例NHL按照WHO新分类进行病理观察和10种抗体免疫表型测定。结果：B系占78．9％，其中弥漫性大B细胞淋巴瘤（DLBL）最多；其次为MALT型（＋／－单核细胞样B细胞）及淋巴浆细胞性淋巴瘤（LPL）。T系占21．1％，以外周T非特殊型最多。CD79a对B－NHL染色阳性率达100％，未见与T细胞的交叉反应。CD3对T－NHL染色阳性率为88％，与B细胞仅有5％的交叉反应。结论：我国沈阳地区NHL以DLBL和外周T非特殊型为最多见，结内单核B和淋巴浆细胞性比例较高。CD79a和CD3分别是具有高度敏感性和特异性的B、T细胞标志抗体。

Classification and Pathological Analysis of Immunophenotype of 76 Cases of Non-Hodgkin Lymphoma in Shenyang

Objective: Our aim was to discuss the pathological characteristics and immunophenotype of non Hodgkin lymphoma (NHL) in Shenyang. Methods: Histopathological observation was performed with immunohistochemical methods (SP method). We used 10 antibodies as B,T markers to analyze 76 cases of NHL according to the new WHO classification. Results: The B NHL was more prevalent than the T NHL. In B NHL, the Diffuse Large B cell Lymphoma (DLBL) was the most common, next was the mucosa associated lymphoid tissue lymphoma ( /-monocytoid B cells) and lymphoplasmacytic lymphoma (LPL). In T NHL, the peripheral T cell lymphoma unspecificly accounted most. The positivity rates of CD 79 a in B NHL were 100% and its cross reactivity was 0. The straining rates of polyclonal CD 3 with T cell were 88% and CD 3 only reacted with 5% of B NHL. Conclusion: The NHL in Shenyang was marked by the most common DLBL and peripheral T cell lymphoma, unspecificly. The rates of monocytoid lymphoma and LPL were higher. The CD 79 a and CD 3 were antibodies of B cell and T cell markers with high sensitivity and specificity, respectively.