A minD mutant of enterohemorrhagic E. coli O157:H7 has reduced adherence to human epithelial cells |
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| Authors: | Parti Rajinder P Biswas Debabrata Wang Monica Liao Mingmin Dillon Jo-Anne R |
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| Affiliation: | aVaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK, Canada S7N 5E3;bDepartment of Biology, University of Saskatchewan, W.P. Thompson Building, 112 Science Place, Saskatoon, SK, Canada S7N 5E2 |
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| Abstract: | ![]()
Adherence to epithelial cells is a prerequisite for intestinal colonization by the bacterial pathogen, enterohemorrhagic Escherichia coli (EHEC). The deletion of minD, a cell division gene, in EHEC caused reduced adherence to human epithelioid cervical carcinoma (HeLa) and human colonic adenocarcinoma (Caco-2) cells as compared to wild-type. The minD mutant formed minicells and filaments owing to aberrant cytokinesis. Moreover, its ability to form microcolonies as typically seen in the co-cultures of wild-type with Caco-2 cells, was abolished. In conclusion, the present study highlights the importance of minD in regards to EHEC adherence to human epithelial cells. |
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| Keywords: | Enterohemorrhagic E. coli O157:H7 Adherence Microcolony minD |
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