小鼠脉络膜血管新生模型中FLK-1的表达

目的:探讨小鼠脉络膜血管新生(choroidal neovascu larization, CNV)模型中FLK-1的表达.方法:采用激光击射的方法诱导成年C57BL/6小鼠CNV模型,术后行脉络膜灌流铺片及组织病理学检查观察CNV的发展.应用免疫组织化学染色探讨正常视网膜以及激光术后10d CNV模型中FLK-1的表达.结果:术后10d所有激光光斑均发展为实验性CNV,其中血管内皮细胞,类视网膜色素上皮细胞(retinal pigmented epithelium,RPE)及类成纤维细胞FLK-1表达强烈;在正常视网膜中,FLK-1仅在RPE、内核层及神经节细胞层有微弱的表达.结论:FLK-1参与了实验性CNV的发病过程,提示拮抗FLK-1可能成为防治CNV有效的生物学方法之一.

Expression of FLK-1 in laser-induced choroidal neovascularization in mouse

AIM:To investigate the expression of vascular endothelial growth factor receptor 2(VEGFR-2,also known as FLK-1) in laser-induced choroidal neovascularization(CNV) in mouse.METHODS:CNV was induced in C57BL/6 mouse eyes by krypton laser photocoagulation.Choroidal fluorescein angiography and histopathological examination were used to assess the development of experimental CNV.Cryostat sections from lesions on day 10 after laser treatment and normal eyes were prepared for Immunohistochemistry for FLK-1.RESULTS:Laser-induced CNV developed in all lesions on day 10.The expression of FLK-1 was detected in endothelial cells,retinal pigmented epithelium(RPE) -like cells and fibroblast-like cells in neovascular lesions.In normal adult mouse retinas,FLK-1 expression was mainly observed in RPE cells,inner nuclear and ganglion cell layers.CONCLUSION:Our findings demonstrated that expression of FLK-1 may play a role in the formation of laser-induced CNV in mice,which suggest that FLK-1 may be a promising potential target for antiangiogenesis therapy for CNV.