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| 力竭运动致大鼠心肌损伤及S100A4蛋白表达变化 | |
| 引用本文： | 刘子泉,陈昀赟,王天辉,徐传香,王德刚,张娜,刘晓海,刘洪涛. 力竭运动致大鼠心肌损伤及S100A4蛋白表达变化[J]. 中国公共卫生, 2011, 27(5) |
| 作者姓名： | 刘子泉 陈昀赟 王天辉 徐传香 王德刚 张娜 刘晓海 刘洪涛 |
| 作者单位： | 1. 军事医学科学院卫生学环境医学研究所军事劳动卫生研究室,天津300050;武警医学院 2. 军事医学科学院卫生学环境医学研究所军事劳动卫生研究室,天津,300050 3. 北京军区卫生干部训练中心 |
| 基金项目： | 国家自然科学基金(30971421); 天津市自然科学基金(10JCYBJC10200) |
| 摘 要： | 目的观察S100A4蛋白在力竭运动损伤心肌组织中的表达变化,探讨S100A4蛋白对力竭运动损伤心肌组织的作用。方法 W istar大鼠12只,随机分为对照组和力竭运动组,大鼠进行力竭训练10d后,取血及心肌组织,用光镜和电镜对心肌进行组织学观察;采用蛋白免疫印迹(western-b lot)法和免疫组织化学法检测心肌蛋白表达。结果力竭运动组大鼠心肌组织苏木精-伊红(HE)染色可见大鼠心肌和血管内皮细胞空泡变性和心肌间质水肿等;电镜可见血管内皮细胞发生典型细胞凋亡特征;W estern-b lot结果表明,对照组心肌中S100A4蛋白弱表达,而力竭运动组S100A4蛋白表达量升高;免疫组化结果显示,与对照组比较,力竭运动组心肌中S100A4蛋白表达升高1.68倍,且在心肌和血管内皮细胞中均有表达。结论力竭运动造成大鼠心肌损伤,S100A4蛋白在损伤心肌中表达升高。 |
| 关 键 词： | S100A4蛋白 力竭运动 氧化应激 心肌 |
Damage and expression of S100A4 in cardiac muscle induced by exhaustion exercise in rat |
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| LIU Zi-quan,CHEN Yun-yun,WANG Tian-hui,et al.. Damage and expression of S100A4 in cardiac muscle induced by exhaustion exercise in rat[J]. Chinese Journal of Public Health, 2011, 27(5) | |
| Authors: | LIU Zi-quan CHEN Yun-yun WANG Tian-hui et al. |
| Affiliation: | LIU Zi-quan,CHEN Yun-yun,WANG Tian-hui,et al.Department of Military Occupational Health,Institute of Hygiene and Enviromental Medicine,Academy of Military Medical Sciences(Tianjin 300050,China) |
| Abstract: | Objective To observe the expression of S100A4 and to study the effect of S100A4 on cardiac muscle injury induced by exhaustion exercise.Methods Twelve male Wistar rats were divided into two groups:control group(CN) and exhaustion exercise group(EI).After 10 days of exhaustion exercise,the rats in both CN and EI were immediately decapitated and their blood and cardiac muscle samples were collected.The histology of cardiac muscle were detected with light microscope and transmission electron microscope.The exp... |
| Keywords: | S100A4 protein exhaustion exercise oxidative stress cardiac muscle |
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