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| 氟对大鼠骨组织Osterix mRNA及其蛋白表达影响 | |
| 引用本文： | 杨丹,于燕妮,邓超男,朱海振. 氟对大鼠骨组织Osterix mRNA及其蛋白表达影响[J]. 中国公共卫生, 2011, 27(8) |
| 作者姓名： | 杨丹 于燕妮 邓超男 朱海振 |
| 作者单位： | 贵阳医学院病理教研室,贵州,贵阳,550004 |
| 基金项目： | 贵阳市科学技术项目(2009筑科农合同字第3-007); 科技部国际合作重点项目(2010DFB30530) |
| 摘 要： | 目的观察氟对大鼠股骨中成骨细胞特异性转录因子Osterix(OSX)mRNA及其蛋白表达的影响。方法 36只SD大鼠按体重随机分为对照组、低氟组(饮水含氟50 mg/L)、高氟组(饮水含氟5 mg/L),饲养6个月后股动脉放血处死。用免疫组织化学法检测各组大鼠股骨组织中OSX蛋白表达;实时荧光定量PCR检测OSX mRNA表达。结果高氟组大鼠OSX阳性成骨细胞数[(51.47±3.37)个]增多,明显高于低氟组[(36.80±2.83)个]和对照组[(27.00±2.92)个],差异有统计学意义(P<0.05);低氟组OSX mRNA是对照组的2.10倍,高氟组OSX mRNA是对照组的2.82倍,与对照组比较,差异有统计学意义(P<0.05)。结论氟可导致大鼠骨组织OSX mRNA及蛋白表达水平增高,OSX可能参与氟引起的骨骼损伤的发生机制。 |
| 关 键 词： | OSX 氟中毒 骨组织 实时荧光定量PCR |
Effect of fluoride on expression of OSX mRNA and protein in bone tissue of rats |
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| YANG Dan,YU Yan-ni,DENG Chao-nan,et al.. Effect of fluoride on expression of OSX mRNA and protein in bone tissue of rats[J]. Chinese Journal of Public Health, 2011, 27(8) | |
| Authors: | YANG Dan YU Yan-ni DENG Chao-nan et al. |
| Affiliation: | YANG Dan,YU Yan-ni,DENG Chao-nan,et al.Department of Pathology,Guiyang Medical University(Guiyang 550004,China) |
| Abstract: | Objective To investigate the changes of mRNA and protein expressions of osterix(OSX) in the bone of rats with chronic fluorosis.Methods Thirty-six SD rats were randomly divided into three groups(12 in each group with half male and half female):control group,low and high fluoride group.The fluoride groups exposed to sodium fluoride(low:NaF of 5 mg/L and high:NaF of 50 mg/L,respectively)added to the drinking water to produce the animal model of fluorosis.The expressions of OSX protein and OSX mRNA inside the ... |
| Keywords: | OSX fluorosis bone real-time quantitative PCR |
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