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| 人口腔鳞癌耐药细胞株中药干预差异性蛋白表达 | |
| 引用本文： | 欧冰凝,唐海桦,张海英,梁钢,韦艳. 人口腔鳞癌耐药细胞株中药干预差异性蛋白表达[J]. 中国公共卫生, 2011, 27(7) |
| 作者姓名： | 欧冰凝 唐海桦 张海英 梁钢 韦艳 |
| 作者单位： | 1. 广西医科大学药学院天然药化教研室、广西南宁530021 2. 广西医科大学药学院药理教研室 3. 广西医科大学公共卫生学院 |
| 基金项目： | 国家自然科学基金(30760310); 广西自然科学基金(桂科自0728113) |
| 摘 要： | 目的筛选中药成分干预人口腔鳞癌耐药细胞株KBV200前后差异性表达蛋白质。方法采用四甲基偶氮噻唑蓝(MTT)法检测5种中药对KBV200的细胞毒性,选择对细胞抑制率低于10%(IC10),即无细胞毒作用的浓度,联合200 ng/mL长春新碱(VCR)对KBV200细胞株进行干预;取干预前后的细胞总蛋白提取液,用表面加强激光解析电离-飞行时间质谱(SELD I-TOF-MS)和CM-10蛋白质芯片技术筛选差异性蛋白。结果质荷比2 000～50 000Da范围内,表没食子儿茶素没食子酸酯(EGCG)干预KBV200前后共捕获13个差异蛋白质峰(均P<0.05),对于3997、4941、4968、5971、6084 Da 5个蛋白质峰,人口腔鳞癌细胞株KB表达较低,而KBV200表达明显升高(P<0.05),EGCG干预后表达明显降低(P<0.05);大黄素干预KBV200前后共捕获到6个差异蛋白质峰(P<0.05),对于4968,5971,6084 Da 3个蛋白质峰,KB表达较低,KBV200表达明显升高(P<0.05),大黄素干预后表达明显降低(P<0.05);未检测到两面针碱、板蓝根粗提物和岩黄连粗提物干预... |
| 关 键 词： | 口腔鳞癌 KBV200 SELDI-TOF-MS 中药 |
Differential protein expression in KBV200 cell lines before and after treatment with traditional Chinese medicines |
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| OU Bing-ning,TANG Hai-hua,ZHANG Hai-ying,et al.. Differential protein expression in KBV200 cell lines before and after treatment with traditional Chinese medicines[J]. Chinese Journal of Public Health, 2011, 27(7) | |
| Authors: | OU Bing-ning TANG Hai-hua ZHANG Hai-ying et al. |
| Affiliation: | OU Bing-ning,TANG Hai-hua,ZHANG Hai-ying,et al.Pharmaceutical College of Guangxi Medical University(Nanning 530021,China) |
| Abstract: | Objective To examine differential protein expression in drug-resistast human oral squamous carcinoma cell lines KBV200 before and after the treatment with five traditional Chinese medicines.Methods The cytotoxicity of traditional Chinese medicines to KBV200 cells were determined by methyl thiazol tetrazolium(MTT) assay.The different protein expression profiles in KBV200 cells were detected by surface enhanced laser desorption /ionization-time of flight-mass spectrometry(SELDI-TOF-MS) and CM-10 chip before a... |
| Keywords: | oral squamous carcinoma KBV200 SELDI-TOF-MS traditional Chinese medicine |
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