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| 应用序列特异引物PCR法对广西壮族HLA－DQA_1进行基因分型 | |
| 引用本文： | 龙桂芳,阿卜迪,李卫,林伟雄,唐智宁,谢建生,金琪. 应用序列特异引物PCR法对广西壮族HLA－DQA_1进行基因分型[J]. 中华血液学杂志, 1995, 0(10) |
| 作者姓名： | 龙桂芳 阿卜迪 李卫 林伟雄 唐智宁 谢建生 金琪 |
| 作者单位： | 广西医科大学第一附属医院儿科血红蛋白实验室 |
| 摘 要： | 应用序列特异引物多聚酶链反应（ＰＣＲ）方法对８２名无血缘关系的广西壮族健康人的ＨＬＡ－ＤＱＡ＿１进行基因分型。共检出７种ＤＱＡ＿１等位基因，以ＤＱＡ＿１＊０３０１的频率最高，达３１％，其次为ＤＱＡ＿１＊０１０４，０１０２和０５０１，检出率分别为２４．３％，１６．９％和１１．７％。未检出的等位基因有ＤＱＡ＿１＊０２０１，０３０２和０６０１。结果表明，壮族的ＨＬＡ－ＤＱＡ＿１等位基因频率分布除与中国南方人相似外，尚有其特点。序列特异引物ＰＣＲ方法不需要放射性同位素，简便快速、准确可靠的ＨＬＡ－ＤＱＡ＿１基因分型新方法。 |
| 关 键 词： | 多聚酶链反应，序列物异引物，基因分型，HLA－DQA＿1 |
HLA-DQA1 GENOTYPING FOR GUANGXI ZHUANGNATIONALITY BY THE PCR-SSP TECHNIQUE |
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| Long Guifang, AM Abdi, LiWei,et al.. HLA-DQA1 GENOTYPING FOR GUANGXI ZHUANGNATIONALITY BY THE PCR-SSP TECHNIQUE[J]. Chinese Journal of Hematology, 1995, 0(10) | |
| Authors: | Long Guifang AM Abdi LiWei et al. |
| Affiliation: | Long Guifang, AM Abdi, LiWei, et al. |
| Abstract: | LA typing for DQA1 locus in 82 unrelated,ran-domly selected,healthy individuals of Guangxi ZhuangNationality was carried out.Seven officially recognizedalleles were detected,of which DQA1 * 0301 occurdwith the highest frequency of 3 1%,Other alleles ofhigh frequency included DQAi * 0104 ,0102 and 0501with frequencies of 24.3%, 16.9%and 11.7%,re-spectively, However, DQA_1 * 0201, 0302 and 0601were not detected.Our results indicated that the HLA-DQA_1 of Zhuang Nationality were not only similar tothose of southern Chinese populations,but also exhib-ited their own unique features. PCR-SSP technique is anon-radioactive,accurate and reliable method for HLAgenotyping. |
| Keywords: | HLA-DOA1 Genotyping Poly-merase chain reaction |
| 本文献已被 CNKI 等数据库收录！ | |