应用大孔吸附树脂吸附分离技术制备蒲黄总黄酮的研究

考察13种大孔吸附树脂对蒲黄总黄酮的吸附分离性能,确定大孔吸附树脂吸附分离蒲黄总黄酮的工艺条件.以总黄酮吸附量、总黄酮含量和总黄酮回收率为考察指标,测定总黄酮含量采用紫外-可见分光光度法,考察泄露曲线采用薄层扫描法.结果表明AB-8型树脂对蒲黄总黄酮有良好吸附分离性能,其吸附分离蒲黄总黄酮的工艺条件为:蒲黄提取物上样浓度为20 mg/mL,蒲黄总黄酮最大吸附量为9.36 mg/mL,吸附流速为2 mL/min,洗脱剂为50%乙醇,洗脱剂用量为7倍量树脂体积,洗脱速度为2 mL/min,树脂可重复使用3次.AB-8型大孔吸附树脂在所确定的工艺条件下,可较好地吸附分离蒲黄总黄酮.其乙醇洗脱物中蒲黄总黄酮含量达45%以上,总黄酮回收率达85%以上.

Preparation of Puhuang (Pollen of Typha angustifolia or Typha orientalis) Total Flavone by the Adsorption and Separation of Macroporous Adsorption Resin

In order to set up the technology for adsorbing and separating total flavone from Puhuang (Pollen of Typha angustifolia or Typha orientalis) with macroporous adsorption resin, 13 types of macroporous adsorption resin were investigated. The total flavone adsorption capacity, total flavone content and total flavone recovery rate were used as the evaluating criteria. The UV-VIS spectrophotometric method was used in the determination of total flavone content, and TLCS was used in the evaluation of the leaking curve. The results showed that among 13 types of macroporous adsorption resin, the AB-8 was the best for adsorbing and separating Puhuang total flavone in the following technological conditions: the concentration of the sample of Puhuang extract was 20 mg/ml; the maximum adsorbing capacity for Puhuang total flavone, 9.36 mg/ml; the current velocity of adsorption, 2 ml/minute; the eluting reagent, 50% ethanol (7 times the volume of the resin); eluting velocity, 2 ml/minute; and the AB-8 resin could be used 3 times repeatedly. The results also showed that AB-8 macroporous adsorption resin could well adsorb and separate Puhuang total flavone under the set technological conditions, and the content of Puhuang total flavone in the ethanol eluant was over 45%, with a total flavone recovery rate of over 85%.