GABA对大鼠下丘脑薄片室旁核神经元放电活动的影响

目的：观察γ-氨基丁酸（GABA）对室旁核神经元（PVN）放电活动的影响，并探讨其作用机理。方法：用玻璃微电极细胞外记录了GABA对60个大鼠脑薄片111个室旁核神经元（PVN）放电活动的影响。结果：GA-BA可以使98个PVN中的73%放电频率明显减少甚至停止，具有浓度依赖性，此反应可以部分被GABAA受体的竞争性拮抗剂荷包牡丹碱所拮抗，另外GABAB受体的选择性激动剂苯氯丁氨酸也可明显抑制PVN的放电活动。结论：GABA对室旁核神经元的放电活动具有明显的抑制作用，是通过GABAA和GABAB受体共同介导的。

Effects of GABA on paraventricular neurons from hypothalamic slices in vitro

objective: Experiments were performed to investigate the effects of GABA on paraventricular neurons from hypothalamic slices and to elucidate the mechanism in vitro. Method: The spontaneous discharges of 111 paraventricular neurons from 60 hypothalamic slices were recorded by glass microelectrode. Results: During the perfusion of GABA (10 -3 mol/L), the discharge rats of 73% out of 98 paraventricular neurons were significantly decreased, even ceased with a shot latency. The GABA induced response, which was concentration dependent, could be partially blocked by the Bic. Baclofen can also inhibit the spontaneous discharges of the PVN. Conclusion: These results suggest that GABA can inhibit obviously the burst activities on PVN neurons and this inhibition is mediated by GABA\-A and can GABA\-B receptor, the GABA\-A and GABA\-B receptor co exist on the same PVN neuron.