在ERCP中应用小剂量氯胺酮复合异丙酚的临床观察

目的探讨小剂量氯胺酮复合异丙酚静脉麻醉在经十二指肠胆道检查治疗技术中的安全性与可行性。方法将80例患者随机均分成两组。麻醉组在异丙酚和氯胺酮复合麻醉下进行检查和治疗；对照组术前肌肉注射哌替啶50mg和地西泮10mg。观察比较两组患者的呼吸循环指标、操作过程评分和镇静评分。结果麻醉组操作评分和镇静评分明显优于对照组，两组术前循环指标无统计学差异，麻醉组术中循环指标与术前比较无统计学差异，但显著性低于常规组的相同时间点（P〈0．05）；常规组术中循环指标显著性高于其术前（P〈0．05）。结论小剂量氯胺酮复合异丙酚的麻醉方法应用于经十二指肠胆道检查治疗术是安全可行的，并且该方法对循环指标不稳定和依从性差的患者具有优越性。

Application of small-dose Katemine and propofol during ERCP

Objective To study the regulation of HCV NS3 protein on the expression of survivin prorein in QSG7701 cell and the interacion with apoptosis for investigating carcinogenesis mechanism of HCV NS3 protein in human liver cell. Methods Apoptosis induced by serum starvation or not, the expressions of survivin protein are detected by Western Blot and immunocytochemistry, the apoptosis indexes are examined by TUNEL.Results HCV NS3 protein activates the expression of survivin in QSG7701 cell. After apoptosis induced by serum starvation, the expressions of survivin are down-regulated. HCV NS3 protein also activates the expression of survivin which exhibits the tendency of time and concentration in QSG7701 cell. At the same time, HCV NS3 protein inhibits apoptosis induced by serum deprivation in QSG7701 cell. Conclusion HCV NS3 protein inhibits apoptosis by up-regulating the expression of survivin protein in QSG7701 cell, contributes to malignant transformation of hepatocytes and hepatocellular carcinogenesis related HCV.