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| 应用聚合酶链反应制备HBV DNA生物素探针 | |
| 引用本文： | 毛申兰,陈常庆,姚光弼. 应用聚合酶链反应制备HBV DNA生物素探针[J]. 上海医学, 1993, 0(12) |
| 作者姓名： | 毛申兰 陈常庆 姚光弼 |
| 作者单位： | 中科院上海生物工程研究中心，中科院上海生物工程研究中心，上海静安区中心医院 |
| 摘 要： | 通过聚合酶链反应将Bio-11-duTP掺入扩增的HBV DNA中,制备了长度为120bp的生物素探针,可检测到0.1pg的HBV DNA。PCR际记法简便迅速,特异性强,所用DNA量少且无需分离纯化,其标记探针得率高,活性强。 |
| 关 键 词： | 聚合酶链反应 乙型肝炎病毒 |
THE RAPID PRODUCTION OF BIOTINYLATED HBV DNA PROBE USING THE POLYMERASE CHAIN REACTION |
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| Mao Shen-lan,et al.. THE RAPID PRODUCTION OF BIOTINYLATED HBV DNA PROBE USING THE POLYMERASE CHAIN REACTION[J]. Shanghai Medical Journal, 1993, 0(12) | |
| Authors: | Mao Shen-lan et al. |
| Affiliation: | Mao Shen-lan,et al. Shanghai Biotechnology Research Center,Chineses Academy of Sciences,Shanghai 200233 |
| Abstract: | By including Bio-11-dUTP during polymerase chain reaction (PCR), HBV DNA probe of very high specific activity has been generated (sensitivity of detection: 0.1pg of HBV DNA). The advantages of PCR labeling include (1) labeling with subnanogram amounts of input DNA; (2) rapid production of vector-free probes; (3) easy regulation of both the specific activity and the amount of labeled probe produced. |
| Keywords: | polymerase chain reaction hepatitis B virus |
| 本文献已被 CNKI 等数据库收录！ | |