STAT3蛋白在肝癌组织中的表达及临床意义

目的探讨STAT3和p-STAT3蛋白在肝癌组织中的表达及其临床意义。方法采用免疫组织化学的方法检测了90例肝癌组织及其相应的癌旁组织中STAT3和p-STAT3蛋白的表达,分析STAT3和p-STAT3蛋白表达与临床病理特征及患者预后的关系。特征参数之间用χ2检验;采用Kaplan-Meier法进行生存分析,用Log-rank检验进行曲线间比较;运用Cox回归进行单因素及多因素生存分析。结果肝癌组织中STAT3主要在细胞质中表达,而p-STAT3主要表达在细胞核内;STAT3和p-STAT3在肝癌组织中阳性表达率明显高于癌旁组织;STAT3和p-STAT3蛋白的表达与卫星灶（P=0.033,P〈0.01）、血管浸润（P=0.046,P〈0.01）及AJCC分期（P〈0.01,P〈0.01）有关,与患者的性别（P=0.280,P=0.403）、年龄（P=0.432,P=0.844）、肿瘤大小（P=0.762,P=0.161）、肿瘤数量（P=0.301,P=0.326）、肿瘤的分化程度（P=0.753,P=0.910）及甲胎蛋白（P=0.441,P=0.080）比较差异无统计学意义;肝癌组织中STAT3和p-STAT3蛋白高表达的患者5年生存率明显低于低表达患者。结论 STAT3和p-STAT3蛋白可能参与了肝癌的浸润转移,有望成为一种新的肝癌预后参考指标。

STAT3 protein expression profile and its clinical significance in hepatocellular carcinoma

Objective To determine the protein expression profiles of STAT3 and its activated form, p -STAT3, in hepatocellular carcinoma （HCC） and investigate the potential clinical significance. Methods The expressions of STAT3 and p -STAT3 were detected in 90 HCC tissues and patient - matched cancer - adjacent healthy tissues by immunohistochemistry. The significance of between - tissue differences in protein expres- sion was assessed by Chi - squared test. The relations of significant differential expression with patients＇ clinieopathological features and prognoses （based on 5 -year follow -up） were assessed by Cox regression models. Five -year survival rates were determined by Kaplan -Meier analysis with Log -rank test. Results In HCC tissues, STAT3 was mainly detected in the cytoplasm and p -STAT3 was mainly detected in the nucleus. The levels of both STAT3 and p - STAT3 were significantly higher in HCC than in adjacent non - cancer tissues. There was no significant relation for ei- ther STAT3 or p - STAT3 protein expression with satellite lesions, vascular invasion, AJCC stage of disease progression, sex, age, tumor size, tumor number, degree of differentiation, or alpha - fetoprotein expression. The 5 - year survival rate of patients with high expressions of STAT3 and p - STAT3 in HCC tissues was significantly lower than patients with low expression. Conclusion STAT3 and p - STAT3 protein may contribute to the invasive and metastatic qualities of HCC, and represent a useful new prognosis reference index of this liver cancer.