淋病菌流行株耐药与耐药与耐药质粒谱的研究

目的 了解淋球菌流行株的耐药状况，并对耐药表型及质粒谱进行分型。方法 1998－1999年从广东省湛江地区分离出98株淋球菌流行株， 采用琼脂稀释法测定淋球菌对青霉素、四环素及环丙沙星的敏感性；应用碘量法测定β－内酰胺酶，并对耐药表型进行分型；应用碱裂解法提取相应菌株的质粒，对质粒谱进行分型。结果 淋球菌流行株对青霉素、四环素及环丙沙星的耐药率分别为22．44％、45．94％和55．12％；β－内酰胺酶阳性淋球菌占6．12％（6／98）；耐药表型以Susceptible^PT、CMRNG^T、CMRNG^PT、TRNG四型为主，分别占43．86％、23．46％、10．20％和10．20％；98株淋球菌流行株，质粒总检出率为91．84％，42．5kb、39．5kb、7．4kb、4．2kb质粒分别占11．22、41．82％、59．16％和67．32％；质粒谱型12型，以7．4kb 4.2kb、39.5kb 7.4kb 4.2kb为主，分别占21．42％和17．34％。结论 湛江地区淋球菌流行株对环丙沙星的耐药率较高，耐药表型和质粒谱型各有其分布特点。

Study on the Resistance and the Resistant Plasmid Profiles in Prevalent Gonococcal Strains

Objective To investigate the resistant status and to type the phenotype and plasmid profiles of prevalent gonococcal strains. Methods A total of 98 gonococcal strains isolated from 1998 to 1999 in Zhanjiang was identified systemically. The susceptibilities of 98 strains of gonococci to penicillin,tetracycline and ciprofloxacin were detected by agar dilution method. The β － lactamase production was tested by iodometric method. The plasmids were extracted by alkaline lysis technique. Results The prevalence of gonococcal strains resistant to penicillin, tetracycline and ciprofloxacin were 22.44％ ,45.94％ and 55.12％ ,respectively. There were 6 positive strains for β － lactamase, accounting for 6.12％ (6/98). The most common phenotypes were susceptiblePT、 CMRNGT、 CMRNGPT and TRNG, accounting for 43.86％ ,23.46％ ,10.20％ and 10.20％ ,respectively. The detection rate of plasmids were 91.84％ .Among them, the percentages of 42.5 kb,39.5 kb,7.4 kb and 4.2 kb plasmids accounted for 11.22％ ,41.82％ ,59.16％ and 67.32％ ,respectively.There were 12 types of plasmid profiles(PP), the predominant types were 7.4kb＋ 4.2kb and 39.5kb＋ 7.4kb＋ 4.2kb, accounting for 21.42％ and 17.34％ ,respectively. Conclusion The gonococcal strains resistant to ciprofloxacin are very high in Zhanjiang. The phenotype and plasmid profiles of the strains have characteristic distribution.