丹皮多糖-2b对2型糖尿病大鼠模型的作用及其降糖作用机制

目的　观察丹皮多糖 2b(PSM 2b)对 2型糖尿病(T2DM)大鼠模型的作用 ,并探讨其降血糖作用的机制。方法　链脲霉素和高热量喂饲复制大鼠T2DM。灌胃给药 5wk。用葡萄糖氧化酶法、放射免疫分析法及受体放射配体结合分析法分别测定血清葡萄糖、胰岛素及肝细胞膜的胰岛素受体。结果　PSM 2b能明显降低空腹血糖 ,改善糖耐量异常及血脂异常 ,提高肝细胞低亲和力胰岛素受体 (InsR)最大结合容量 (RT2 ) ,使胰岛素敏感性指数 (ISI)增加 (P <0 0 5 )。结论　PSM 2b对T2DM大鼠模型有明显的治疗作用 ,其降糖机制与提高胰岛素受体数目、改善受体环节的胰岛素抵抗有关

Effect of polysaccharides-2b from Mudan cortex on type 2 diabetes mellitus rats and its mechanism

AIM To study the effect of polysaccharides 2b from Mudan cortex (PSM 2b) on type 2 diabetes mellitus (T2DM) rats and explore its mechanism. METHODS T2DM rat model was established by low dosage streptozotocin and high sucrose fat diet. The drugs were administrated by ig for 5 weeks. The serum glucose was assayed with GOD POD method. Insulin was determined by radioimmunoassay. Insulin receptors of the plasma membrane from rat liver were determined with radioligand binding assay of receptors (RBAR). RESULTS PSM 2b could significantly lower fasting blood glucose (FBG), improve impaired glucose telarance (IGT) and dyslipidemia. It could also remarkably raise receptor total (RT2) of the low affinity insulin receptor (InsR) and insulin sensitivity index (ISI) in T2DM rats. CONCLUSION PSM 2b has an obvious therapeatic effect on T2DM in rats. Its mechamsm is relatively improve insulin resistance (IR) in T2DM rats by raising the number of InsR.