SLE患者血清及外周血单个核细胞中IL-12、IL-18的检测及临床意义

目的探讨系统性红斑狼疮（SLE）患者IL-12、IL-18的表达及与疾病活动的关系。方法应用ELISA法以及半定量RT-PCR法分别测定SLE活动组、缓解组和对照组血清IL-12、IL-18水平以及外周血单个核细胞中的IL-12 mRNA和IL-18 mRNA的表达。结果SLE活动组IL-18 mRNA、IL-18的表达较SLE缓解组和正常对照组明显升高（P均〈0.05）。血清IL-18水平与IL-12水平呈负相关（r=-0.406,P=0.019）。SLE患者SLE疾病活动指数与IL-12、IL-12 mRNA呈负相关（r=-0.617,P〈0.05;r=-0.512,P〈0.05）,与IL-18、IL-18 mRNA呈正相关性（r=0.817,P〈0.05;r=0.806,P〈0.01）。结论SLE活动组患者中存在IL-18异常高表达和IL-12异常低表达,二者之间呈负相关,在SLE发病和发展中可能起不同作用。

Expressions of IL-12 and IL-18 in blood serum and peripheral blood mononuclear cells in patients with systemic lupus erythematosus

Objective To investigate the expressions level of IL-12 and IL-18 in systemic lupus erythematosus（SLE） and their relationships with the activity of diseases.Methods The levels of IL-12 and IL-18 in blood serum and the IL-12 mRNA and IL-18 mRNA in peripheral blood mononuclear cells（PBMC） in SLE active group,SLE remission group and control group were separately measured by ELISA and semi-quantitative RT-PCR.Results The expression of IL-18 mRNA in PBMC and the level of IL-18 in blood serum of SLE active group were significantly higher than those of SLE remission group and healthy control group（all P0.05）.There were significantly negative correlations between the level of IL-18 and IL-12 in blood serum（r=-0.406,P=0.019）.The expressions of IL-12 mRNA and IL-12 correlated negatively with SLEDAI score（r=-0.617,P0.05;r=-0.512,P0.05）.the expressions of IL-18 mRNA and IL-18 correlated positively with SLEDAI score（r=0.817,P0.05;r=0.806,P0.01）.Conclusions The high expression of IL-18 and the low expression of IL-12 in SLE.IL-18 and IL-12 may play different roles in the pathogenesis of SLE.