乙酰胆碱对大鼠体液免疫应答的影响

本文利用神经药理学的方法分别改变大鼠中枢和外周乙酰胆碱(Ach)的含量,以及阻断中枢M或N型的胆碱能受体,观察大鼠的初次体液免疫应答的变化,从而探讨Ach对体液免疫功能的影响及其影响机制。结果表明;(1)侧脑室注射Ach的合成阻断剂密胆碱后,体液免疫应答增强;侧脑室注射Ach酯酶的抑制剂新斯的明后,免疫应答减弱;(2)腹腔给予密胆碱或新斯的明,免疫应答都无明显改变;(3)侧脑室注射M型胆碱能受体拮抗剂阿托品,使体液免疫应答增强;注射N型胆碱能受体拮抗剂六烃季胺则抑制了免疫应答。以上结果提示:中枢神经系统内的Ach可影响体液免疫功能,可能具有免疫抑制效应;外周Ach可能不参与体液免疫功能的调节。中枢Ach的免疫调节作用是通过相应的胆碱能受体实现的。

Influence of Acetylcholine on the Humoral Immune Response in Rat

Author investigated the influence of acetylcholine (Ach) on the primary humoral immune response to sheep red blood cells (SRBC) .The rtsults were as followi (1) Hemicholinium-3, which inhibited the biosynthesis of Ach, markedly enhanced the immune response and neostigmine, the inhibitor of cholinesterase, reaulted in the suppression of the immune response, when they were injected intracerebroventricularly, (2)Intraperitoneal in jection of hemicholinium-3 or neostigmine did not significantly affect the immune response, (3) The immune response was increased following intrace-rebriventricular administration of atropine,a muscarinic antagonist, but was suppressed after administration of hexamethonium, a nicotinic antagonist. These results suggested that Ach in central nervous system can affect the primary humoral immune response to SRBC in rats, but Ach in periphery may not affect the immune response.Furthermore, the immunomodulation by Ach in central nervous system may be mediated by its relevant receptors.