黏附肽改性纯钛表面成骨细胞的早期黏附能力研究

目的 对纯钛表面钝化态氧化膜进行生物化学活化改性,探讨生物化学改性对钛表面成骨细胞黏附的影响.方法 24个纯钛试件打磨抛光后均分为4组,每组6个;纯钛组不处理,碱-热处理组行碱-热处理,溶胶涂层组行碱-热处理+溶胶涂层处理,黏附肽组行碱-热处理+溶胶涂层+黏附精氨酸-甘氨酸-天冬氨酸-丝氨酸肽(Glycine-Tyrosine-Arginine-Glycine-Asparticacid-Serine,GYRGDS)处理.使用X射线光电子能谱(X-ray photoelectron spectroscopy,XPS)、傅里叶变换红外光谱计(Fourier transform infrared spectrometer,FTIR)分析各组钛表面化学元素及功能基团组成;在各组钛试件表面接种成骨细胞,经2.05 Pa流体剪切力作用30 min、1 h、2 h,计算细胞残留率.结果 碱-热处理组钛表面富含大量活性羟基,溶胶涂层组钛表面富含大量羟氧基团.黏附肽组钛表面经XPS及FTIR分析检测到酰胺峰,显示GYRGDS肽成功固定于钛表面.流体剪切力作用30 min、1 h和2 h后黏附肽组钛表面的成骨细胞残留率(分别为93.0%、54.4%、34.4%)均高于相应时间点其他组.结论 GYRGDS肽修饰后的钛表面成骨细胞黏附稳定性均优于非GYRGDS肽修饰组.

Osteoblastic early attachment onto the surface of bioadhesive peptide modified pure titanium

Objective To investigate the long-term integrity and the biological function of interface between the bioadhesive peptide modified implant surface and peri-implant tissue. Methods A short bioadhesive peptide containing Glyeine-Tyrosine-Arginine-Glycine-Asparticacid-Serine(GYRGDS) sequence was immobilized onto the titanium implant surface by means of sol-gel coating technique and self-assembled monolayers(SAM). The chemical composition and organic functional groups on the titanium surfaces were characterized using XPS (X-ray photoelectron spectroscopy ) and FTIR (Fourier transform infrared spectrometer). The adhesive strength and stability of osteoblasts on various implant surfaces were compared under flow condition. Results The results showed that alkali/hot water aging treatment could apparently improve the content of -OH functional groups of titanium surface. The chemical reactive Ti-O-Ti bonding at the surface of titanium played a vital role in inducing the formation of organosilane SAM. GYRGDS peptide can be covalently grafted onto the surface of titanium by SAM technique. The resistance of freshly adherent osteoblasts to detachment by flow was shear time dependent. When the four groups were compared under the same flow stress condition(2.05 Pa) at three specific time spans(30 min, 1 h, 2 h), the cells retention rates in GYRGDS-grafted groups were 93.0%, 54. 4%, 34. 4% respectively and were much higher than those in non-coated groups. Conclusions It was suggested that GYRGDS might have positive effects on maintaining stability and adherence of cells onto the substrates under flow condition.