VE，VA对梭曼致大鼠过氧化损伤的保护作用

目的　研究梭曼（soman）对大鼠急性中毒的自由基损伤作用及VE/VA的抗氧化保护作用. 方法　雄性大鼠40只,按体质量随机分为阴性组（正常对照组）、阳性组（损伤对照组）,VE组（加VE保护组）和VA组（加VA保护组）. 观察每组大鼠血清、大脑、肝脏中VE、总抗氧化力（T-AOC）和一氧化氮合成酶（NOS）的变化. 结果　梭曼中毒后,T-AOC水平、VE含量下降,NOS活性提高. 中毒前后大鼠肝脏中VE质量分数从(0.83±0.02) mg.g-1下降至(0.45±0.06) mg.g-1;T-AOC从(122.7±14.0) mol.s-1.kg-1下降至(70.4±5.4) mol.s-1.kg-1;NOS从(19.6±2.4) mol.s-1.kg-1上升至（39.3±5.5） mol.s-1.kg-1,有显著差异(P<0.05). 而VE/VA较有效地抑制其变化. 结论　梭曼中毒有明显的过氧化损伤作用,VE/VA对梭曼中毒有一定的抗氧化保护作用.

Protective effects of VE/VA against free radical injury induced by soman in rats

AIM　To study the effects of free radical injury induced by soman in rats and antioxidation of VE/VA. METHODS　40 male rats were randomly divided into 4 groups according to weights, i.e., negative group, positive group, VE group and VA group. The serum, cerebrums and liver VE, NOS and T-AOC (total antioxidative capacity) were determined. RESULTS　The VE contents, T-AOC level decreased and NOS activities increased after soman intoxication. In liver, VE contents decreased from (0.83±0.02) mg.g-1 to (0.45±0.06) mg.g-1 and T-AOC level decreased from (122.7±14.0) mol.s-1.kg-1 to (70.4±5.4) mol.s-1.kg-1 (P<0.05), and NOS activities increased from (19.6±2.4) mol.s-1.kg-1 to（39.3±5.5） mol.s-1.kg-1 (P<0.05). And the VE/VA inhibited the change effectively.CONCLUSION　There is mechanism of free radical injury by soman and protective effect of VE/VA on toxic rats.