肺结核患者杀伤细胞免疫球蛋白样受体基因多态性分析

目的探讨杀伤细胞免疫球蛋白样受体(KIRs)基因多态性与肺结核(TB)的关系。方法采用序列特异性引物聚合酶链反应(SSP-PCR),分析109例肺结核患者(TB组)和30例健康者(对照组)中14个KIR基因多态性。TB组又分为30例痰涂片抗酸杆菌阳性(SP组)和79例涂片阴性患者(SN组)。结果与对照组比较,SP组KIR2DL5(P=0.007)、2DS1(P=0.038)、2DS3(P=0.010)、3DS1(P=0.002)阳性率增高,KIR2DS5频率降低(P=0.038);SN组KIR2DL5(P=0.018)、2DS1(P=0.000)、2DS5(P=0.000)、3DS1(P=0.001)阳性率增高;KIR2DS4(P=0.004)和KIR2DS5(P=0.000)频率SP组较SN组升高;患者携带两个及以上活化KIR的频率较对照组明显升高(P=0.013);患者组基因型AJ的频率较对照组显著降低。结论 KIR基因多态性可能与肺结核的易感性有关。

Polymorphisms of killer cell immunoglobulin-like receptor genes in patients with pulmonary tuberculosis

Objective To investigate association of polymorphisms of killer cell immunoglobulin-like receptor(KIR) genes with susceptibility to tuberculosis(TB) infection.Methods The sequence specific primer polymerase chain reaction(SSP-PCR) method was used to analyze 14 KIR genes in genomic DNA in 30 smear-positive(SP) patients,79 smear-negative(SN) patients and 30 healthy controls.Results Frequencies of KIR2DL5(P=0.007),2DS1(P=0.038),2DS3(P=0.010) and 3DS1(P=0.002) were statistically significantly higher in the SP group than in the control group,while KIR2DS5(P=0.038) was lower.Also,frequencies of KIR2DL5(P=0.018),2DS1(P=0.000),2DS5(P=0.000) and 3DS1(P=0.001) were significantly higher in the SN group than in the control group.Compared with the SN group,frequencies of KIR2DS4(P=0.004) and KIR2DS5(P=0.000) were higher in the SP group.In addition,the number of subjects carrying more than two activating KIR genes in the TB group was higher than that in the control group(P=0.013).The frequency of genotype AJ was lower in TB patients than in the controls.Conclusion Polymorphisms of KIR genes may be associated with susceptibility to TB infection.