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| 活性氧生成在8-硝基白杨素诱导人胃癌SGC-7901细胞凋亡中的作用 | |
| 引用本文： | 蒋崇辉,艾小红,杨小红,罗琼,曹建国.活性氧生成在8-硝基白杨素诱导人胃癌SGC-7901细胞凋亡中的作用[J].山东大学学报(医学版),2012,50(2):34-37. |
| 作者姓名： | 蒋崇辉 艾小红 杨小红 罗琼 曹建国 |
| 作者单位： | 1. 衡阳市中心医院药剂科,湖南衡阳42100 2. 南华大学第一附属医院肿瘤内科,湖南衡阳,421001 3. 湖南师范大学医学院,长沙,410013 |
| 基金项目： | 湖南省自然科学基金项目(03JJY5009);湖南省卫生厅医药科研项目(C20050) |
| 摘 要： | 目的研究活性氧生成在8-硝基白杨素(NOC)诱导人胃癌SGC-7901细胞凋亡中的作用。方法体外培养SGC-7901细胞,采用流式细胞术分析细胞凋亡率,ELISA法检测细胞组蛋白/DNA碎片,H2DCFH-DA探针流式细胞仪检测细胞活性氧(ROS)生成。结果 NOC诱导亚G1峰(Sub-G1)细胞百分率增高和细胞组蛋白/DNA碎片增加(P<0.01),促进SGC-7901细胞活性氧生成(P<0.01)。抗氧化剂N-乙酰半胱氨酸(NAC)能有效对抗NOC诱导SGC-7901细胞凋亡作用。结论 NOC诱导SGC-7901细胞凋亡作用与促进活性氧生成相关。 |
| 关 键 词： | 胃肿瘤 白杨素 白杨素衍生物 凋亡 活性氧 |
Generation of reactive oxygen species in apoptosis of human gastric cancer SGC-7901 cells induced by 5,7-dihydroxy-8-nitrochrysin |
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| JIANG Chong-hui , AI Xiao-hong , YANG Xiao-hong , LUO Qiong , CAO Jian-guo.Generation of reactive oxygen species in apoptosis of human gastric cancer SGC-7901 cells induced by 5,7-dihydroxy-8-nitrochrysin[J].Journal of Shandong University:Health Sciences,2012,50(2):34-37. | |
| Authors: | JIANG Chong-hui AI Xiao-hong YANG Xiao-hong LUO Qiong CAO Jian-guo |
| Institution: | 1.Department of Pharmacy,Hengyang Central Hospital,Hengyang 421001,Hunan,China; 2.Department of Oncology,First Affiliated Hospital of Nanhua University,Hengyang 421001,Hunan,China; 3.School of Medicine,Hunan Normal University,Changsha 410013,China) |
| Abstract: | Objective To investigate induction of apoptosis and generation of reactive oxygen species(ROS) of human gastric cancer SGC-7901 cells in vitro by 5,7-dihydrox-8-nitrochrysin(NOC).Methods SGC-7901 cells were cultured in vitro and the percentage of Sub-G1 cell population was detected by PI stained flow cytometry(FCM).Cell histone-associated DNA fragments were examined by ELISA assay.The intracellular accumulation of ROS was determined by FCM using the fluorescent probe H2DCFDA.Results NOC significantly increased the percentage of Sub-G1 cell population and histone-associated DNA fragments in SGC-7901 cells in a concentration-dependent manner(P<0.01).NOC also markedly promoted accumulation of ROS(P<0.01).The pre-incubation of N-acetylcysteine,an antioxidant,effectively antagonized the induction of apoptosis of SGC-7901 cells by NOC.Conclusion NOC induced apoptosis of the SGC-7901 cell line is associated with induction of ROS generation. |
| Keywords: | Gastric neoplasms Chrysin Chrysin derivatives Apoptosis Reactive oxygen species |
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