甘草酸二胺联合前列地尔对大鼠肾间质纤维化的影响

目的 观察甘草酸二胺联合前列地尔对大鼠肾间质纤维化的影响.方法 建立单侧输尿管梗阻(UUO)动物模型,将120只大鼠随机分为假手术组、模型组、甘草酸二胺组、前列地尔组、甘草酸二胺加前列地尔组,每组24只.术后第7、14、21天每组各处死8只大鼠,测定其血清肌酐(CREA)水平,HE染色后观察肾间质变化,免疫组化法观察肾间质转化生长因子-β1(TGF-β1)和结缔组织生长因子(CTGF)的表达,酶联法测定血清TGF-β1、CTGF浓度.结果 模型组在术后第14、21天时血清CREA、TGF-β1、CTGF浓度及TGF-β1、CTGF在肾组织的表达较假手术组均显著增加(P均＜0.01)；甘草酸二胺加前列地尔组的肾间质损伤指数及TGF-β1、CTGF在肾间质的表达和血清中TGF-β1、CTGF的浓度均明显低于其他治疗组(P均＜0.01).结论 甘草酸二胺联合前列地尔能改善UUO所致的肾间质纤维化,其机制可能与下调肾组织的TGF-β1、CTGF表达有关.

Effect of combination therapy of glycyrrhizin acid and prostaglandin E1 on renal interstitial fibrosis in rats

Objective To investigate the effect of glycyrrhizin acid(GA) and prostaglandin E1(PGE1) on renal interstitial fibrosis(RIF) in rats.Methods Rat models of unilateral ureteral obstruction(UUO) were established by ligating left ureteral.120 rats were randomly divided into 5 groups: sham group,model group,GA group,PGE1 group and GA+PGE1 group.8 rats in each group were sacrificed at days 7,14 and 21 after operation.The serum level of creatinine(CREA) was determined,and the histopathological examination of renal tissue were observed by HE staining under optical microscope.The serum levels of transforming growth factor β1(TGF-β1) and connective tissue growth factor(CTGF) were detected by ELISA.The expressions of CTGF and TGF-β1 in renal tissue were observed by immunohistochemical staining.Results In model group,histopathological scores,levels of serum CREA,TGF-β1 and CTGF,and the expressions of TGF-β1 and CTGF were all significantly increased than those in sham group on days 14 and 21 after operation(P<0.01).In GA+PGE1 group,the degree of renal interstitial damage,and serum contents and expressions of TGF-β1 and CTGF were remarkably reduced compared with those in other treatment groups(P<0.01).Conclusion GA and PGE1 have potent preventive effect on RIF in UUO rats,which may be related to down-regulation of TGF-β1 and CTGF.