Glu-GNPs对人肺腺癌细胞株A549放射增敏的初步探讨

目的探讨偶联葡萄糖的纳米金颗粒(Glu-GNPs)对人A549细胞的放射增敏作用及其机制。方法噻唑蓝(MTT)法检测低浓度(≤20 nmol/L)Glu-GNPs联合放射对A549细胞存活的影响,克隆形成检测Glu-GNPs对A549细胞的放射增敏作用,流式细胞仪(FCM)检测细胞周期及其凋亡。结果低浓度Glu-GNPs对A549细胞生长无明显抑制,联合X射线后具有抑制作用,在15 nmol/L范围内,随浓度增大,抑制作用增强;15 nmol/LGlu-GNPs对A549细胞有放射增敏作用,由Dq、Do计算放射增敏比(SER)分别为1.93、1.10;Glu-GNPs、单纯放射均可诱导细胞凋亡,凋亡率分别为(7.64±1.43)%、(13.46±1.99)%,联合放射组凋亡率为(21.43±1.04)%,显著高于前两组(P<0.01);Glu-GNPs作用后,细胞周期发生变化,表现为S期减少,G2/M期增加(P<0.05)。结论 Glu-GNPs对人肺腺癌细胞株A549具有放射增敏作用,其机制可能为抑制细胞亚致死损伤修复,阻滞细胞于G2/M期,并诱导细胞凋亡。

Radiosensitivity enhancement of the human lung adenocarcinoma cell line A549 by Glu-GNPs

Objective To investigate radiosensitivity enhancement and its mechanism of thio-glucose bound gold nanoparticles(Glu-GNPs) on the lung adenocarcinoma cell line A549.Methods MTT assay was used to determine the effect of Glu-GNPs in low concentrations(≤20 nmol/L) combined with radiotherapy on the survival of A549 cells.The radiosensitivity enhancement effect of Glu-GNPs on A549 cells was measured by clonal formation.The cell cycle and apoptosis were assayed by the flow cytometric method(FCM).Results Survival of A549 cells was not obviously restrained by Glu-GNPs in low concentrations.But it was inhibited by Glu-GNPs combined with X-ray,and the inhibition was enhanced with the increase of concentrations within 15 nmol/L.According to Dq and Do,sensitization enhancement ratios(SER) of A549 cells were 1.93 and 1.10,respectively.Radiation and Glu-GNPs could both induce apoptosis,and their apoptosis rates were(13.46±1.99)% and(7.64±1.43)%,respectively,which were enhanced to(21.43±1.04)% by combined treatment(P<0.01).Glu-GNPs decreased A549 cells at the phase S and increased those at G2/M(P<0.05).Conclusion Glu-GNPs could induce radiosensitivity enhancement on the lung adenocarcinoma cell line A549,and the mechanism may be related to restraining repair of sub-lethal damage,blocking cells at G2/M and inducing apoptosis.