EV71临床分离株BALB/c小鼠致病特点分析

目的 将肠道病毒71型(EV71)临床分离株接种小鼠观察其致病特点.方法 将6株EV71临床株分别腹腔接种1日龄BALB/c小鼠,观察小鼠表现以及脑、肺脏、骨骼肌组织病理变化;采用免疫组化和RT-PCR方法,检测组织中病毒抗原和基因片段.结果 临床分离的6株EV71(C4亚型)接种1日龄小鼠均出现病症,其中JN200804株实验小鼠症状较重,小鼠脑组织和骨骼肌可观察到细胞空泡变性、淋巴细胞浸润等病理变化;JN200804株感染组小鼠脑内检测到EV71抗原和基因片段.结论 6株EV71临床株均可感染小鼠并致病,重者出现松弛型麻痹并死亡,轻者出现震颤、麻痹但可恢复至正常.

Analysis of the pathogenic characteristics of BALB/c mice infected with enterovirus 71 isolates

Objective To study the characteristics of enterovirus 71(EV71)isolates in vivo,and to provide information for the research of EV71.Methods One-day old BALB/c mice were infected with 6 EV71 strains isolates,and symptoms were observed.All mice were sacrificed at 7d after the infection to collect organs and tissues for the pathological examination,RT-PCR detection and immunohistochemistry.Results 6 viruses were isolated from stool samples,and identified as C4 genotype of EV71.All of them could induce lesions of one-day old mice.The JN200804 strain induced flaccid paralysis on mice 5 days after the infection.Histopathologically,lymphocytic infiltration and some other pathologic changes were observed in the infected mice brain,lung and skeletal muscles.mRNA and antigens was detected in the brain of mice infected with JN200804.Conclusion 6 strain of EV71 isolates can cause the disease on BALB/c mice,including the paralysis,muscular tremor,and death of infected mice.