基于抗病毒活性检测的板蓝根质量生物评价方法及优化研究

目的 建立基于抗病毒活性检测的板蓝根质量生物评价方法并进行优化研究。方法 分别采用红细胞凝集活性检测法和流感病毒神经氨酸酶（NA）活性检测法，建立板蓝根抗病毒活性生物测定方法，并对所建立的两种方法进行对比分析与优选。结果 两种方法可用于对不同样品进行活性检测和区分，结果重复性较好（RSD＝7.0%、5.78%）；Pearson相关性分析表明板蓝根凝集活性与NA抑制活性之间具有显著相关性（P＜0.01，r＝?0.81），进一步印证了凝集活性测定法与抗病毒药理作用的关联性；在安全性、经济性、简便性和实用性等方面凝集活性检测法优势明显，可作为板蓝根质量生物测定的优选方法。结论 本研究优选凝集活性检测法用于板蓝根质量的生物测定，与抗流感病毒药效相关，提高了其现行质量控制水平，并为中药质量生物测定方法的研究提供参考。

Bio-evaluation methods and optimization for Isatidis Radix quality control based on antiviral activity detection

Objective To establish and optimize a bio-evaluation method based on antiviral activity detection for Isatidis Radix quality control. Methods According to the fact that antivirus activity is the main pharmacological effect of Isatidis Radix, the means of hemagglutination activity detection and the means of influenza virus neuraminidase (NA) activity detection were used for establishing two antiviral activity detection methods for the bio-evaluation of Isatidis Radix. In addition, contrastive analyzation and optimization of the two methods were carried out in this study. Results The two methods could be used to evaluate and distinct the biological activities of different batches of Isatidis Radix samples and both methods have good repeatabilities (RSD = 7.0% and 5.78%). The result shows a good correlation (P<0.01, r = ?0.81) between the neuraminidase inhibitory activity and the hemagglutination activity of Isatidis Radix. The relationship between agglutination activity and antiviral effect of Isatidis Radix was further verified. Agglutination detection method has the superiority to its safety, inexpensiveness, easiness, and practicality. Conclusion This novel method could reflect the pharmacodynamic activity of Isatidis Radix on anti-influenza virus and be helpful for the quality control of Isatidis Radix. This study provides reference for the bio-evaluation on the quality control of Chinese materia medica.