经肝动脉灌注平阳霉素碘油乳剂对兔正常肝脏组织的影响

目的：探讨平阳霉素碘油乳剂（PLE）经肝动脉灌注对兔正常肝脏组织的影响。方法：14只4～5个月龄日本大耳白兔，体重（2.5±0.2）kg，按注入生理盐水或PLE的量分为假手术组、实验A组（低剂量组）和实验B组（高剂量组）。各组兔均开腹穿刺肝动脉，按分组剂量注入PLE。术后1，2，4，6周取病理切片，HE染色，光镜下观察肝脏组织学改变;免疫组织化学（免疫组化）染色标记血小板衍化生长因子B链（PDGF-B），并行图象分析。结果：A组HE染色肝细胞呈一过性水样变，变性在2周时最重，至6周已明显减轻。B组HE染色2周时肝细胞明显水样变，4周时可见汇管区纤维组织增生，6周时部分肝组织出现明显假小叶结构。免疫组化染色显示，PDGF-B在肝细胞胞膜及纤维间隔中有明显表达。结论：经肝动脉灌注PLE可导致正常肝脏组织产生不同程度的肝纤维化;PDGF-B参与了肝纤维化的病理过程。

The effect of hepatic intra-arterial injection of emulsions of lipidol and pingyangmycin on the rabbit normal hepatic tissues

Objective To explore the effect of hepatic intra-arterial injection of emulsions of lipidol and pingyangmycin (EL+P) on normal hepatic tissues. Methods Forteen big-ear white rabbits were divided into three groups: sham-operation group, A group (low dose of EL+P),and B group(high dose of EL+P). Laparotomy was performed, and various doses of EL+P were perfused into the hepatic artery. Biopsy specimens were obtained on the 1st, 2nd, 4th, and 6th week respectively after the operation. Hematoxylin-eosin stain, immunohistochemistry stain of platelet derived growth factor B(PDGF-B) and quantitative analysis were performed. Results The main manifestation of A group was transient hepatocyte hydropic degeneration; in group B the degeneration of hepatocytes was most severe in the 2nd week. Fibrosis appeared in the 4th week,and obvious pseudolobules were formed in some parts of the liver in the 6th week. The expression of PDGF-B was mainly concentrated on the membrane of hepatocytes and fibrotic septum. Conclusions Hepatic intra-arterial injection of EL+P can result in damage of normal hepatic tissue. PDGF-B participated in the process of hepatic fibrosis.