| 微小隐孢子虫子孢子与宿主黏附相关蛋白的筛选 |
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| 引用本文： | 郭安,尹继刚,向梅,刘贤英,张岩,陈启军. 微小隐孢子虫子孢子与宿主黏附相关蛋白的筛选[J]. 中国寄生虫学与寄生虫病杂志, 2009, 27(1): 87-88 |
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| 作者姓名： | 郭安 尹继刚 向梅 刘贤英 张岩 陈启军 |
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| 作者单位： | 1吉林大学人兽共患病研究所人兽共患病教育部重点实验室,长春 130062;2 吉林大学第二临床医院妇产科,长春 130041 |
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| 摘 要： | 利用人结肠癌细胞（Caco-2 cell）对微小隐孢子虫子孢子噬菌体展示文库进行筛选。对筛选得到的噬菌体进行克隆及测序分析,共获得5个特异的基因片段,其中1个为已知的编码微小隐孢子虫重要的侵入和黏附相关蛋白CP2,另外4个编码功能未知的蛋白。筛选得到的蛋白可能参与子孢子与宿主细胞的黏附过程。
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| 关 键 词： | 微小隐孢子虫 T7噬菌体展示 筛选 黏附 |
Screening for relevant proteins involved in adhesion of Cryptosporidium parvum sporozoites to host cells |
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| GUO An,YIN Ji-gang,XIANG Mei,LIU Xian-ying,ZHANG Yan,CHEN Qi-jun. Screening for relevant proteins involved in adhesion of Cryptosporidium parvum sporozoites to host cells[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2009, 27(1): 87-88 |
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| Authors: | GUO An YIN Ji-gang XIANG Mei LIU Xian-ying ZHANG Yan CHEN Qi-jun |
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| Affiliation: | 1 Key Laboratory of Zoonosis,Ministry of Education,Institute of Zoonosis,Jilin University,Changchun 130062,China;2 Department of Obstetrics and Gynecology,The Second Hospital,Jilin University,Changchun 130041,China |
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| Abstract: | The Cryptosporidium parvum T7 phage display library was screened by using Caco-2 cells. Five specific gene fragments were identified by blasting sequences in GenBank, one of which encoding the CP2 protein was previously identified as a surface molecule of sporozoites and involved in parasite invasion. The others are hypothetic proteins with unknown functions. Bioinformatic analysis of these proteins indicated that they may be involved in the host-parasite interactions. |
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| Keywords: | Cryptosporidium parvum')" >Cryptosporidium parvum T7 phage display Screening ')" >Adhesion |
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