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| HPLC法测定活血通脉片中人参皂苷Rg_1的含量 | |
| 引用本文： | 张邦升,张志健,袁本香. HPLC法测定活血通脉片中人参皂苷Rg_1的含量[J]. 中国药房, 2007, 18(24): 1880-1881 |
| 作者姓名： | 张邦升 张志健 袁本香 |
| 作者单位： | 1. 银川市第二人民医院药剂科,银川市,750011 2. 西安政法学院门诊部,西安市,710061 3. 宁夏医学院药检系,银川市,750021 |
| 摘 要： | 目的:建立以高效液相色谱法测定活血通脉片中人参皂苷Rg1含量的方法。方法:色谱柱为SHIP-PACK VP ODS(150mm×4.6mm,5μm),流动相为乙腈-水(28:72),流速为1.0mL.min-1,检测波长为210nm,柱温为25℃,进样量为20μL。结果:人参皂苷Rg1检测浓度在40.0～200.0μg.mL-1范围内与峰面积积分值呈良好的线性关系(r=0.9999);平均加样回收率为99.95%,RSD=6.78%(n=6)。结论:本方法可靠、灵敏、准确、易行,可用于活血通脉片的质量控制。 |
| 关 键 词： | 高效液相色谱法 活血通脉片 人参皂苷Rg1 含量测定 |
| 文章编号： | 1001-0408（2007）24-1880-02 |
| 收稿时间： | 2006-11-03 |
| 修稿时间： | 2006-11-032007-01-04 |
Determination of Ginsenoside Rg1 in Huoxuetongmai Tablets by HPLC |
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| ZHANG Bangsheng,ZHANG Zhijian,YUAN Benxiang. Determination of Ginsenoside Rg1 in Huoxuetongmai Tablets by HPLC[J]. China Pharmacy, 2007, 18(24): 1880-1881 | |
| Authors: | ZHANG Bangsheng ZHANG Zhijian YUAN Benxiang |
| Abstract: | OBJECTIVE:To establish a HPLC method for the determination of the content of Ginsenoside Rg1 in Huoxue tongmai tablets.METHODS:The analytical column was SHIP-PACK VP ODS(150mm× 4.6mm,5μ m) with column temperature at 25℃,and the mobile phase was composed of acetonitrile-water(28:72) with flow rate at 1.0 mL· min-1.The detection wavelength was set at 210nm and the sample size was 20μ L.RESULTS:The linear range of Ginsenoside Rg1 was 40.0～ 200.0μg· mL-1(r=0.999 9),and its average recovery was 99.95%,RSD=6.78%(n=6).CONCLUSION:This method is reliable,sensitive,accurate and easy to operate,and it can be used as a quality control of Huoxue tongmai tablets. |
| Keywords: | HPLC Huoxue tongmai tablets Ginsenoside Rg, Content determination |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |