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Effect of erythropoietin on the expression of HIF-1 and iNOS in retina in chronic ocular hypertension rats |
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| Authors: | Dong-Mei Gui Yang Yang Xun Li Dian-Wen Gao |
| Affiliation: | Department of Ophthalmology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, Liaoning Province, China,Department of Ophthalmology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, Liaoning Province, China,Department of Ophthalmology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, Liaoning Province, China and Department of Ophthalmology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, Liaoning Province, China |
| Abstract: | AIMTo research the effect of erythropoietin (EPO) to the HIF-1iNOS signal transduction path in retina in chronic ocular hypertension rat.METHODSOne hundred and twenty Wistar rats were divided into 12 groups randomly. Two episcleral veins were coagulated unilaterally in rats with electric coagulator to establish the glaucoma model. PT-PCR and Western Blot analysis were used to examine the expression of Caspase-9 genes in retina. And the changes of ERG-b wave before and after were detected using EPO.RESULTSIn EPO drug treatment group, the amplitude of ERG-b wave of retina restored remarkably. There was significant difference between two groups (P<0.05). The expressions of HIF-1iNOS mRNA and protein in EPO drug treatment group were weakened remarkably. It was statistically different compared with the non-drug treatment group.CONCLUSIONOne of protect mechanisms of EPO to injured retina caused by chronic intraocular hypertension is through HIF-1iNOS signal conduct path. |
| Keywords: | EPO HIF-1 iNOS ocular hypertension retina |
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