鸡骨草醇提物体外抗氧化自由基作用研究

目的 测定鸡骨草(Herba Abri)色素的抗活性氧自由基活性作用，为充分利用鸡骨草资源提供理论依据。方法 以65%乙醇作为溶剂，pH=3条件下浸泡24 h，利用旋转蒸发器减压蒸发辅助法提取鸡骨草色素；通过紫外分光光度法测定鸡骨草色素清除超氧自由基(?O2-)和羟自由基（?OH）能力﹑清除1，1-二苯基-2-三硝基苯肼(DPPH)自由基能力、普鲁士法测定其还原Fe3 能力、以及以丁基羟基茴香醚（butylated hydroxylanisole， BHA）作对照测定鸡骨草色素对金属离子Fe2 螯合能力。结果 鸡骨草色素具有较强的清除超氧自由基(?O2-)、羟自由基（?OH）、DPPH自由基能力，还原Fe3 能力和Fe2 螯合能力。结论 鸡骨草色素具有较强抗氧化作用，其抗氧化性随着浓度的增大而增强。

Study on the in Vitro Antioxidant Activity of the Ethanol Extracts from Abri Herba

OBJECTIVE To study in vitro antioxidant activity of the ethanol extracts from Abri Herba, and provide the theory to make use of Abri Herba. METHODS Abri Herba was soaked for 24 h with 65% ethanol as solvent and under the condition of pH=3, the abilities of the ethanol extracts of clearing ·O^2-, ·OH, and DPPH were assayed by spectrophotometry. The ability of reducing Fe³⁺ was detected with Prussia methodand, and chelating Fe2+ was detected comparison to BHA. RESULTS The ethanol extracts of Abri Herba could effectively clear ·O^2-, ·OH and DPPH, and reduce Fe³⁺, chelate Fe²⁺. CONCLUTION The ethanol extracts of Abri Herba antioxidation is strong, and its antioxidant activity was enhanced with the increase of concentration.