As2O3和全反式维甲酸对人宫颈癌HeLa细胞生长的影响

目的 探讨As2O3和全反式维甲酸(ATRA)对人宫颈癌HeLa细胞体外生长的影响及其机制.方法 采用不同浓度As2O3和ATRA分别作用于人官颈癌HeLa细胞,观察细胞形态;采用四甲基偶氮唑蓝(MTT)比色法检测细胞生长情况;半定量逆转录聚合酶链反应(RT-PCR)和Westernblot法检测细胞中N-myc下游调节基因1(NDRG1)mRNA和蛋白的表达.结果 As2O3和ATRA能够抑制宫颈癌HeLa细胞生长,12.5 μmol/L As2O3处理HeLa细胞48 h的抑制率为(36.5±1.2)%,明显高于同时间低浓度组;1 × 10-5mol/LATRA处理HeLa细胞48 h的抑制率为(23.5±3.1)%,明显高于同时间低浓度组,并且同浓度的药物处理72 h的抑制率高于处理48 h的抑制率,呈浓度和时间依赖性(P<0.05).As2O3和ATRA能够从分子水平和蛋白水平上调宫颈癌HeLa细胞中NDRG1基因的表达,12.5 μmol/L As2O3和5×10-6mol/L ATRA处理HeLa细胞后,NDRG1 mRNA表达量分别为0.942±0.169和0.894±0.138,明显高于低浓度组的表达量;12.5 μmol/LAs2O3和5×10-6 mol/LATRA处理HeLa细胞后,NDRG1蛋白表达量分别为1.220±0.202和1.233±0.103,明显高于低浓度组的蛋白表达量.结论 As2O3能够抑制宫颈癌HeLa细胞生长,其抑制作用与NDRG1基因的表达上调相关.

Abstract：

Objective To study the effect of arsenic trioxide (As2O3 ) and all-trans retinoic acid ( ATRA ) on human cervical carcinoma HeLa cell line.Methods HeLa cells were treated with As2O3 and ATRA.The cell proliferation was evaluated by MTT assay.The expressions of NDRG-1 protein and mRNA were determined by Western blot and RT-PCR analysis.Results MTT assay showed that As2O3 and ATRA inhibited the growth of human cervical carcinoma HeLa cells in vitro in a dose- and time-dependent manner.Western blot and RT-PCR techniques showed that As2O3 and ATRA down-regulated the expressions of NDRG-1 protein and mRNA (P < 0.05 ) .Conclusion As2O3 and ATRA can significantly inhibit the growth and proliferation of HeLa cells.The reason of these changes may be related with the down-regulation of expression of NDRG-1.

Effects of As2O3 and all-trans retinoic acid on the growth of HeLa cell line and their relation with gene NDRG1

Objective To study the effect of arsenic trioxide (As2O3 ) and all-trans retinoic acid ( ATRA ) on human cervical carcinoma HeLa cell line.Methods HeLa cells were treated with As2O3 and ATRA.The cell proliferation was evaluated by MTT assay.The expressions of NDRG-1 protein and mRNA were determined by Western blot and RT-PCR analysis.Results MTT assay showed that As2O3 and ATRA inhibited the growth of human cervical carcinoma HeLa cells in vitro in a dose- and time-dependent manner.Western blot and RT-PCR techniques showed that As2O3 and ATRA down-regulated the expressions of NDRG-1 protein and mRNA (P < 0.05 ) .Conclusion As2O3 and ATRA can significantly inhibit the growth and proliferation of HeLa cells.The reason of these changes may be related with the down-regulation of expression of NDRG-1.