燃煤型氟中毒大鼠海马区组织病理学分析

目的 复制燃煤型氟中毒大鼠模型并观察其对海马CA1区形态学影响.方法 24只SD大鼠随机分为3组,对照组、低、高氟组；染氟组以地氟病区燃煤烘烤的玉米为主要饲料,复制氟中毒动物模型,6个月后用氟离子选择电极法检测动物尿、骨及脑氟含量,观察大鼠海马CA1区神经元病理变化,用Biornias2000图象分析系统测试大鼠海马CA1区神经元胞体平均面积、周长及平均灰度值.结果 低、高氟组大鼠尿氟为(2.87 ±0.12)、(5.27±0.15)mg/L,骨氟(2 912±140)、(4 624±20) mg/kg,脑氟(1.14±0.04)、( 1.79 ±0.04) mg/kg;与对照组尿氟(1.68±0.02)mg/L、骨氟(1479±127) mg/kg、脑氟(0.52±0.05) mg/kg比较,差异有统计学意义(P＜0.05或P＜0.01)；尼氏染色显示,与对照组大鼠神经元平均灰度值( 115±4)比较,染氟组大鼠均明显增加低氟(157±7),高氟(164±8)].结论 长期食用燃煤型氟中毒病区燃煤烘烤的粮食可以引起氟中毒；并导致动物尿、骨、脑氟含量增高,使大鼠海马CA1区神经元蛋白合成功能下降.

Histopathological changes in hippocampal CA1 area of rats with coal burning fluorosis

Objective To duplicate the animal model with coal burning fluorosis and to observe its influence on the histopathology in the hippocampal CA1 area in rats.Methods Twenty-four SD rats were randomly divided into control,low-fluoride,and high-fluoride exposed groups.Low-and high-fluoride exposed groups were fed with the corn polluted in drying processes with burning coal containing high level of fluoride from the endemic fluorosis area to produce the animal model of fluorosis.During the sixth month experiment,...