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| 维生素A缺乏对大鼠铁调节蛋白2影响 | |
| 引用本文： | 姜珊,王朝旭,姜丽英,李丹娜. 维生素A缺乏对大鼠铁调节蛋白2影响[J]. 中国公共卫生, 2011, 27(10) |
| 作者姓名： | 姜珊 王朝旭 姜丽英 李丹娜 |
| 作者单位： | 哈尔滨医科大学公共卫生学院营养与食品卫生学教研室,黑龙江哈尔滨,150086 |
| 基金项目： | 国家自然科学基金，黑龙江省自然科学基金 |
| 摘 要： | 目的 探讨维生素A缺乏对大鼠铁调节蛋白2(IRP2)mRNA及铁蛋白(Fn) nRNA和转铁蛋白受体(TfR)mRNA表达影响.方法48只雄性SD大鼠按体重随机分为4组,每组12只,对照组、维生素A完全缺乏组；维生素A边缘缺乏组；铁及维生素A边缘缺乏组；喂养8周后,麻醉处死大鼠,取组织和血清,进行相关指标和基因表达检测.结果与对照组( 1.50 +0.41) μmol/L比较,维生素A完全缺乏大鼠血清维生素A(0.22±0.26) μmol/L水平明显降低(P＜0.05)；与对照组比较,维生素A完全缺乏组大鼠血清铁(2.26 +0.72)lμg/mL明显降低；维生素A完全缺乏组大鼠肝脏IRP2、TfR表达水平分别为(1.53±0.18)、(0.62±0.06)与对照组相比明显升高,FnmRNA (0.52±0.08),明显低于对照组(P＜0.05).结论维生素A缺乏通过改变IRP2表达、IRP-RNA结合活性,在转录后水平改变TfR和Fn mRNA表达,影响铁稳态. |
| 关 键 词： | 维生素A缺乏 铁代谢 铁调节蛋白 铁蛋白 转铁蛋白受体 |
Effect of vitamin A deficiency on iron regulatory protein 2 in rats |
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| JIANG Shan,WANG Chao-xu,JIANG Li-ying,et al.. Effect of vitamin A deficiency on iron regulatory protein 2 in rats[J]. Chinese Journal of Public Health, 2011, 27(10) | |
| Authors: | JIANG Shan WANG Chao-xu JIANG Li-ying et al. |
| Affiliation: | JIANG Shan,WANG Chao-xu,JIANG Li-ying,et al.Department of Nutrition and Food Hygiene,School of Public Health,Harbin Medical University(Harbin 150086,China) |
| Abstract: | Objective To explore the potential role of iron regulator protein 2(IRP2) under conditions of vitamin A deficiency and the relationship between vitamin A deficiency and anemia.Methods Forty-eight SD rats were divided randomly into four groups of control,complete deficient in vitamin A,marginal deficiency in vitamin A,and ion plus vitamin A marginal deficiency.After 8 weeks,all the rats were killed by sodium pentobarbital anesthesia and all samples were collected and detected for gene expression.Results As e... |
| Keywords: | vitamin A deficiency iron metabolism iron regulatory protein ferrintin transferrin receptor |
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