螺内酯对2型糖尿病大鼠24小时尿白蛋白排泄率的影响

目的]观察螺内酯对2型糖尿病大鼠肾功能的影响.方法]48只大鼠用高脂高糖饲料喂养2个月后,腹腔注射 30 mg/kg链脲佐菌素制备糖尿病大鼠模型.随机分为糖尿病组 (DM组,n =24)、螺内酯治疗组(R组,n =24),另选同批普通饲养大鼠15只作为正常对照组 (C组,n=15),检测第16周末血清中血糖(FBG)、糖化血红蛋白(HbA1c)、钾(K+)、24 h尿白蛋白排泄率(UAER)、尿素氮(U-BUN)、肌酐(Cr)、甘油三酯(TG).并用He染色的方法观察16周后大鼠肾脏的病理学变化.结果]第16周的血糖水平DM组和 R组相近,无显著差别( P >0.05),但DM组和 R组的血糖水平均显著高于A组( P <0.01);UAER水平DM组和 R组显著高于C组( P <0.01),且R组UAER水平显著低于DM组( P <0.01).结论] 螺内酯能通过降低糖尿病大鼠UAER,改善糖尿病大鼠的肾功能从而发挥保护肾脏的作用.

Effect of Spironolactone on 24h Urine Albumin Excretion Rate in Rats with Type 2 Diabetes Mellitus

Objective] To observe the effect of spironolactone on renal function in rats with type 2 diabetes mellitus （T2DM）. Methods]The rat model of T2DM was prepared by intraperitoneal injection of streptozocin（STZ） 30mg/kg after feeding high-sugar and fat diet for 2 months. The rats were assigned to diabetic group（group DM, n ：24） and spi- ronolactone treatment group（group R, n = 24）. Other 15 rats fed with normal diet were selected as normal control group （group C, n = 15）. Blood glucose（FBG）, glycosylated hemoglobin（HbAlc）, kalium（K＋）, 24h urine albumin excretion rate（UAER）, urea nitrogen（U-BUN）, creatinine（Cr） and triglyceride（TG） at the end of 16th week were detected. HE staining was used to observe the pathological change of kidney in rats after 16 weeks. Results] At the end of 16th week, BG level in group DM was similar to that in group R, and there was no significant difference（ P 〉0.05）. BG level in group DM and group R were significantly higher than that in group C（ P 〈0.01）. UAER level in group DM and group R were significantly higher than that in group C（ P〈0. 001）, but UAER level in group R was significantly lower than that in group DM（ P〈0.01）. Conclusion] Spironolactone may play the protective role for kidney through decreasing UAER level and improving renal function in diabetic rats.