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Long-term study of TNBS-induced colitis in rats: Focus on mast cells
Authors:A. Menozzi  C. Pozzoli  E. Poli  M. Lazzaretti  D. Grandi  G. Coruzzi
Affiliation:(1) Department of Human Anatomy, Pharmacology and Forensic Medicine, Section of Pharmacology, University of Parma, via Volturno 39, 43100 Parma, Italy;(2) Section of Human Anatomy, University of Parma, via Gramsci 14, 43100 Parma, Italy
Abstract:
Objective and design To investigate the severity and duration of colitis induced by two different doses of 2,4,6-trinitrobenzenesulfonic acid (TNBS) and the changes in mast cell number in acute inflammation and in the recovery process of colitis. Methods Colitis was induced in rats by an enema of TNBS (10 or 30 mg) in 25% ethanol. Macroscopic and histologic changes of the colon, colon weight and mast cell counts were examined at various times (7, 30 and 60 days) after colitis induction. Results TNBS induced a colonic damage which was dose-related for both severity and time necessary to complete recovery. On day 7 after colitis induction 10 mg TNBS induced macroscopic and microscopic alterations of colonic architecture that completely resolved at day 60. By contrast, 30 mg TNBS induced massive necrosis, thickening of the colon, severe histologic changes that were only partially reversed after two months. Mast cell number in the submucosa and muscularis propria decreased significantly in the acute phase of inflammation (7 days) and slowly increased thereafter, reaching a maximum level (up to about 5-fold) at day 60 after both doses of TNBS. Conclusions Present data confirm the ability of TNBS to induce in rats damage to the colon that was dose-dependent for severity and duration. Moreover, these data unravel a different role of mast cells in TNBS-induced colitis: an early degranulation in the acute phase of inflammation and a subsequent accumulation of mast cells in the late phase of the disease, associated with tissue repair. Received 31 January 2006; returned for revision 29 April 2006; accepted by A. Falus 3 May 2006
Keywords:Inflammatory bowel disease  Experimental colitis  2,4,6 trinitrobenzenesulfonic acid (TNBS)  Mast cells
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