| HPLC同时测定麦冬中5个皂苷成分的含量 |
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| 引用本文： | 吴发明,蔡晓洋,王盼,包小红,李敏,周娟. HPLC同时测定麦冬中5个皂苷成分的含量[J]. 中国中药杂志, 2015, 40(20): 4022-4025 |
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| 作者姓名： | 吴发明 蔡晓洋 王盼 包小红 李敏 周娟 |
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| 作者单位： | 成都中医药大学中药材标准化教育部重点实验室, 中药资源系统研究与开发利用国家重点实验室, 四川 成都 611137,成都中医药大学中药材标准化教育部重点实验室, 中药资源系统研究与开发利用国家重点实验室, 四川 成都 611137,成都中医药大学中药材标准化教育部重点实验室, 中药资源系统研究与开发利用国家重点实验室, 四川 成都 611137,四川省食品药品检验检测院, 四川 成都 611000,成都中医药大学中药材标准化教育部重点实验室, 中药资源系统研究与开发利用国家重点实验室, 四川 成都 611137,四川省食品药品检验检测院, 四川 成都 611000 |
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| 基金项目： | 四川省中医药管理局重点项目(2014F038);科技惠民计划项目(2013GS510102);十二五"中药质量安全检测和风险控制技术平台"(2014ZX09304307-002);四川省科技计划项目(2015SZ0033) |
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| 摘 要： | 建立测定麦冬药材中麦冬皂苷D,麦冬皂苷D', ophiopojaponin C,去乙酰基ophiopojaponin A, 3-O-α-L-鼠李糖-(1→2)-β-葡萄糖麦冬苷元5种成分的HPLC方法。采用HPLC-ELSD方法,色谱柱为Kromasil 100-5C18柱(4.6 mm×250 mm,5μm);流动相为乙腈(A)-水(B)溶液,梯度洗脱0~45 min,35%~55%A,体积流量1.0 mL·min-1;柱温35℃,漂移管温度100℃,气体流速3.0 L·min-1;进样量15μL。结果表明5种成分均达到基线分离,各成分均有较宽的线性范围和良好的线性关系(r>0.999);回收率在95.75%~103.1%。建立的麦冬皂苷含量测定方法准确灵敏、重复性好,可应用于麦冬药材质量评价中。
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| 关 键 词： | 麦冬 皂苷 HPLC 含量 |
| 收稿时间： | 2015-05-15 |
HPLC simultaneous determination of contents of 5 saponin constituents in Ophiopogonis Radix |
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| WU Fa-ming,CAI Xiao-yang,WANG Pan,BAO Xiao-hong,LI Min and ZHOU Juan. HPLC simultaneous determination of contents of 5 saponin constituents in Ophiopogonis Radix[J]. China Journal of Chinese Materia Medica, 2015, 40(20): 4022-4025 |
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| Authors: | WU Fa-ming CAI Xiao-yang WANG Pan BAO Xiao-hong LI Min ZHOU Juan |
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| Affiliation: | Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese crudedrug, State Key Laboratory Breeding Base of Systematic Research Development and Utilization of Chinese Medicines Resources Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China,Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese crudedrug, State Key Laboratory Breeding Base of Systematic Research Development and Utilization of Chinese Medicines Resources Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China,Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese crudedrug, State Key Laboratory Breeding Base of Systematic Research Development and Utilization of Chinese Medicines Resources Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China,Sichuan Institute for Food and Drug Control, Chengdu 611130, China,Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese crudedrug, State Key Laboratory Breeding Base of Systematic Research Development and Utilization of Chinese Medicines Resources Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China and Sichuan Institute for Food and Drug Control, Chengdu 611130, China |
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| Abstract: | |
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| Keywords: | Ophiopogonis Radix saponin constituents HPLC content |
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