HBV-M与HBV-DNA关系的研究

目的：研究乙肝5项免疫标志物(HBV-M)与乙肝核酸(HBV-DNA)之间的关系,从而评价检测HBV-DNA载量的临床应用价值。方法：血清HBV-DNA采用美国PE公司生产5700荧光定量PCR仪进行定量检测。5项免疫标志物采用ELISA法。结果表面抗原(HBsAg)、e抗原(HBeAg)和核心抗体(抗-HBc)阳性者,HBV-DNA阳性率为99．5％、e抗体、抗-HBc阳性者,HBV—DNA阳性率为56％;单项HBsAg阳性者,HBV-DNA阳性率为50．1％;HBsAg和抗-HBc阳性者,HBV—DNA阳性率为48．3％;5项免疫标志物皆阴性、含有3个抗体或2个抗体以及注射乙肝疫苗后单项抗-HBs阳性者,HBV—DNA阳性率为0。结论:HBV—DNA载量与抗原存在呈正相关性,而与抗体含量呈负相关性;慢性乙型肝炎患者机体无法清除乙肝病毒,HBV复制是激发机体免疫损害的关键环节,因此抗病毒治疗对减少或阻断肝病的发作和病情进展是十分必要的;HBV—DNA检测对乙肝早期诊断、传染性识别、病毒复制水平判断以及抗病毒治疗效果考核等均有重要的临床意义。

A study on the relationship between HBV-M and HBV-DNA

Objectives To study the relationship between 5 types of immune markers for hepatitis B virus(HBV-M) and HBV-DNA,and to evaluate the clinical significance of determining HBV-DNA.Methods Serum HBV-DNA was determined by PCR assay while 5 types of HBV-M by ELISA methods.Results Of patients positive for HBsAg,HBeAg and Anti-HBc,the HBV-DNA positive rate was 99.5%. Of patients positive for HBeAb and Anti-HBc,the HBV-DNA positive rate was 56%.Of patients positive for HBsAg,and Anti-HBc,the HBV-DNA positive rate was 48.3%.HBV-DNA positive rate was 0 in patients negative for 5 types of immune markers,containing three or two types of antibodies and positive for Anti-HBs after injection with hepatitis B vaccine.Conclusion There was a positive good correlation between carrying capacity of HBV-DNA and the antigens content.Instead,a negative correlation was observed between carrying capacity of HBV-DNA and the antibody content.HBV-DNA determination can be used to monitor the true state of HBV infection and replication,and it is useful for judging effectiveness of Anti-virus treatment. [