HIV感染长期不进展者与艾滋病患者HIV-1 gag特异性CD+8 T细胞应答

目的探讨欧美流行的人类免疫缺陷病毒(HIV)-1 B亚型株与我国HIV感染和艾滋病(AIDS)病人 gag特异性CD+8 T细胞应答交叉反应性.方法研究对象为长期不进展者(LTNP)7例和艾滋病患者9例,将覆盖HXB2 HIV-1 gag全长的125个重叠肽段组成11个肽段库作为抗原,用γ干扰素刺激原酶联免疫斑点试验方法检测LTNP和AIDS病人的特异性CD+8 T细胞应答,观察两组病人间的差异及其与CD+4 T细胞和病毒载量的相关性.结果 LTNP组和AIDS组HIV-1 gag特异性CD+8 T细胞应答强度分别为(1212±796)斑点形成细胞数(SFC)/106外周血单个核细胞(PBMC)和(182±203) SFC/106PBMC,识别肽段库的个数(间接反应了细胞毒性T淋巴细胞应答的宽度)分别为3.0±0.8和0.8±0.7,LTNP组显著高于AIDS组.CD+8 T细胞应答的强度和宽度与CD+4 T细胞计数呈正相关,与病毒载量呈负相关.结论欧美流行株与我国病毒株之间具有交叉反应性,HIV-1 gag特异性CD+8 T细胞应答在阻止疾病进展中可能发挥重要作用.

Human immunodeficiency virus-1 gag specific CD8+ T cell responses in long-term nonprogressors and acquired immunodeficiency syndrome patients

OBJECTIVE: To investigate the human immunodeficiency virus (HIV)-1 gag specific CD(8)(+) T cell responses in long-term nonprogressors (LTNP) and acquired immunodeficiency syndrome (AIDS) patients in China. METHODS: 7 LTNP and 9 AIDS patients were included in this study. 11 peptide pools derived from 125 overlapping peptides spanning the full-length of HXB2 gag were used as antigens. HIV-1 specific CD(8)(+) T cell responses in these patients were examined by interferon gamma ELISPOT assay, and investigated the relationship between HIV-1 gag specific CD(8)(+) T cell responses and CD(+) T cell counts and the relationship between specific CD(8)(+) T cell responses and viral loads. RESULTS: HXB2 could induce HIV-1 specific CD(8)(+) T cell responses in Chinese HIV/AIDS patients. The strengths of HIV-1 gag specific CD(8)(+) T cell responses in LTNP and AIDS groups were (1212 +/- 796) SFC/10(6) PBMC and (182 +/- 203) SFC/10(6) PBMC, respectively. The breadths of HIV-1 gag specific CD(8)(+) T cell responses of LTNP group and AIDS group were 3.0 +/- 0.8 and 0.8 +/- 0.7, respectively. The values of these two parometers in LTNP group were significantly higher than that in AIDS group (P < 0.01). There was a positive relationship between the strength or the breadth of HIV-1 specific CD(8)(+) T cell responses and CD(4)(+) T cell counts, whereas there was a negative relationship between strength or breadth of CD(8)(+) T cell responses and viral loads. CONCLUSIONS: There was a cross-reactivity between dominant HIV strain in Europe and America and those in China. HIV-1 gag specific CD(8)(+) T cell responses may play a protection role during disease progression.