贝伐单抗对实验大鼠脑水肿模型治疗作用研究

目的探讨贝伐单抗腹腔全身给药对实验大鼠脑水肿的干预效应。方法将30只大鼠单纯分成正常组、空白组和治疗组。治疗组又分为建模前72 h注射贝伐单抗(包括低、中、高治疗剂量组),及建模后2 h注射贝伐单抗(包括低、中、高治疗剂量组)。随后,观察各组大鼠大体及光镜下病理组织的水肿改变。同时,测量每组动物的脑组织含水量,并对其神经功能进行评分。最后,应用统计学方法做出分析、总结。结果与空白组比较,贝伐单抗治疗组脑缺血损伤后的神经功能缺失症状明显改善,且随着药物浓度的增加而改善(P<0.01)。同时,随着贝伐单抗浓度的增加,脑水肿的含量逐渐减少,脑组织水肿的情况逐渐减轻,而且,对于贝伐单抗存在一定的浓度依赖性(P<0.01)。但术前给药与术后给药比较,差异无统计学意义(P>0.05)。结论贝伐单抗对实验大鼠脑水肿有一定治疗效果,但具体机制及临床应用需要进一步深入研究。

Study on the therapeutic effect of bevacizumab on brain edema in rats model

Objective To observe the intervention effect of bevacizumab on brain edema by intraperitoneal administration in experi-mental rat brain edema models( utility of cerebrovascular temporary occlusion). Methods Thirty rats were divided into normal group,blank group and bevacizumab group(including low,middle and high dose group)after preoperative and postoperative adminis-tration of bevacizumab. Subsequently,the changes of edema of pathological tissues were observed under gross and light microscope. At the same time,the brain tissue water content of each group was measured and the neurological function was evaluated. Finally,statisti-cal methods were applied to make analysis and summary. Results Compared with the blank group,brain ischemia and reperfusion in-jury after neurological deficit symptoms were improved significantly in bevacizumab group,with the increase of drug concentration (P < 0. 01). At the same time,we could see with the increase of concentration of bevacizumab,brain edema decreased gradually,and gradually reduced the brain edema in a certain concentration dependent manner(P < 0. 01). But there was no statistically significant (P > 0. 05)in rats which were given medication before operation and after the operation. Conclusion Bevacizumab has a certain therapeutic effect on brain edema induced by cerebral ischemia-reperfusion injury,but specific mechanism and clinical application needs further experiments.