| 康莱特对HepG2细胞凋亡及其Caspase-3和Survivin表达影响 |
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| 引用本文： | 金鑫,姚良苹,卢云,李昌生.康莱特对HepG2细胞凋亡及其Caspase-3和Survivin表达影响[J].康复与疗养杂志,2012(1):1-3. |
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| 作者姓名： | 金鑫 姚良苹 卢云 李昌生 |
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| 作者单位： | [1]枣庄矿业集团中心医院普外科,山东枣庄277011 [2]青岛大学医学院附属医院肝胆外科 ,山东枣庄277011 [3]潍坊寿光市人民医院,山东枣庄277011 |
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| 基金项目： | 山东省优秀中青年科学家科研奖励基金资助项目（2006BS03039） |
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| 摘 要： | 目的观察康莱特注射液对HepG2肝癌细胞凋亡及半胱氨酸天冬氨酸蛋白酶-3（Caspase-3）和存活素（Survivin）表达的影响,并初步探讨其作用机制。方法分别采用体积分数0.005、0.010、0.015、0.020的康莱特体外培养HepG2细胞。采用MTT法检测HepG2细胞抑制率,流式细胞仪检测HepG2凋亡情况以及Caspase-3和Survivin表达的变化。结果康莱特能明显抑制HepG2肝癌细胞生长,且呈时间和浓度依赖性。康莱特组中Caspase-3、Survivin蛋白的表达与对照组比较,差异有显著性（P〈0.01）。结论康莱特可抑制HepG2肝癌细胞增殖,并可通过提高Caspase-3蛋白表达和降低Survivin蛋白表达诱导HepG2肝癌细胞凋亡。
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| 关 键 词： | 康莱特 HepG2细胞 细胞凋亡 半胱氨酸天冬氨酸蛋白酶3 Survivin |
EFFECTS OF KANGLAITE INJECTION ON APOPTOSIS OF HEPG2 CELLS AND EXPRESSIONS OF CASPASE-3 AND SURVIVIN |
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| Authors: | JIN Xin YAO Liangping LU Yun LI Changsheng |
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| Institution: | (Department of General Surgery,The Central Hospital of Zaozhuang Coal Mining Group,Zaozhuang 277011,China) |
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| Abstract: | Objective To investigate the effects of Kanglaite injection（KLT） on apoptosis of hepatoma carcinoma cell HepG2（HepG2）,expressions of Caspase-3 and Survivin,and discuss their mechanisms.Methods HepG2 cells were cultured in vitro with different concentrations of KLT（0.005,0.010,0.015,and 0.020）,cell inhibition rate was detected by MTT method,and apoptosis of HepG2,expressions of Caspase-3 and Survivin were determined by using flow cytometry.Results KLT obviously inhibited the growth of HepG2,manifesting time-and concentration-dependent.In KLT group,the differences of expressions of Caspase-3 and Survivin were significant versus the control group（P0.01）.Conclusion KLT can inhibit HepG2 cell multiplication,and induce apoptosis of HepG2 by increasing expression Caspase-3 and decreasing the expression of Survivin. |
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| Keywords: | Kanglaite HepG2 cell apoptosis Caspase 3 Survivin |
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