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HPLC法测定活血通络片中三七皂苷R_1、人参皂苷Rg_1和Re的含量
引用本文:姚育法,吴燕红. HPLC法测定活血通络片中三七皂苷R_1、人参皂苷Rg_1和Re的含量[J]. 中国药房, 2008, 19(27): 2140-2142
作者姓名:姚育法  吴燕红
作者单位:1. 南方医科大学珠江医院,广州市,510515
2. 广东药学院,广州市,510224
摘    要:
目的:建立以高效液相色谱法测定活血通络片中三七有效成分含量的方法。方法:色谱柱为Agilent C18(150mm×4.6mm,5μm),流动相为乙腈-0.05%磷酸水溶液(梯度洗脱),流速为1mL.min-1,检测波长为203nm。结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Re的进样量分别在0.38~3.80μg(r=0.9996)、0.94~9.40μg(r=0.9995)、0.15~1.50μg(r=0.9998)范围内与各自峰面积积分值呈良好线性关系;三者的平均回收率分别为99.23%、97.56%、107.32%,RSD分别为4.35%、4.94%、4.77%。结论:本方法简便、可行、重现性好,可用于活血通络片的质量控制。

关 键 词:活血通络片  高效液相色谱法  三七皂苷R_1  人参皂苷Rg_1  人参皂苷Re  含量测定

Determination of Notoginsenoside R1,Ginsenoside Rg1 and Ginsenoside Re in Huoxue Tongluo Tablets by HPLC
YAO Yu-fa,WU Yan-hong. Determination of Notoginsenoside R1,Ginsenoside Rg1 and Ginsenoside Re in Huoxue Tongluo Tablets by HPLC[J]. China Pharmacy, 2008, 19(27): 2140-2142
Authors:YAO Yu-fa  WU Yan-hong
Affiliation:YAO Yu-fa,WU Yan-hong(1Zhujiang Hospital Affiliated to Southern Medical University,Guangzhou 510515,China;2Guangdong Pharmaceutical College,Guangzhou 510224,China)
Abstract:
OBJECTIVE: To establish HPLC method for the determination of effective components in Huoxue tongluo tablets by HPLC. METHODS: The contents of Notoginsenoside R1, Ginsenoside Rg1 and Ginsenoside Re were simultaneously determined by a HPLC on Agilent C18(150 mm × 4.6 mm, 5μm) column, with mobile phase consisted of acetonitrile- 0.050/0 H3PO4(gradient elution) at a flow rate of 1.0 mL · min^-1 and a detection wavelength of 203 nm.RESULTS: The linear ranges of Notoginsenoside R1, Ginsenoside Rg1 and Ginsenoside Re were 0.38- 3.80μg( r = 0. 999 6), 0.94- 9.40μg( r = 0. 999 5), 0.15-1.50μg( r = 0. 999 8), respectively, their average recoveries were 99.23%, 97.56% and 107.32%, respectively, and the corresponding RSD were 4.35%, 4.94% and 4.77%, respectively.CONCLUSION:The results indicate that the HPLC method is simple, feasible and reproducible, and it is applicable for the quality control of Huoxue tongluo tablets.
Keywords:Huoxue tongluo tablets  HPLC  Notoginsenoside R1  Ginsenoside Rg1  Ginsenoside Re  Content determination
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