| 雌兔阴蒂海绵体平滑肌细胞的体外培养及生物学特性 |
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| 引用本文： | 刘波,刘继红,王涛. 雌兔阴蒂海绵体平滑肌细胞的体外培养及生物学特性[J]. 中华男科学杂志, 2004, 10(3): 198-201 |
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| 作者姓名： | 刘波 刘继红 王涛 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院泌尿外科,湖北,武汉,430030 |
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| 摘 要： | 目的 :探讨雌兔阴蒂海绵体平滑肌细胞的体外培养方法及其生物学特性。 方法 :采用酶消化法对阴蒂海绵体平滑肌细胞进行体外培养 ,倒置显微镜下观察细胞形态 ,计数细胞贴壁率及生长情况 ,用免疫组织化学方法鉴别培养的平滑肌细胞。 结果 :体外培养的细胞证实为兔阴蒂海绵体平滑肌细胞 ,梭形 ,呈长轴平行排列 ,贴壁快 ,生长迅速而平稳。 结论 :体外培养的阴蒂海绵体平滑肌细胞可在合适的条件下生长和传代 ,并能够保持稳定的生物学特性 ,可为阴蒂生理等方面的研究提供细胞材料
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| 关 键 词： | 阴蒂海绵体 平滑肌细胞 体外培养 兔 雌性 |
| 文章编号： | 1009-3591(2004)03-0198-03 |
| 修稿时间： | 2003-12-02 |
Biological Characteristics of Female Rabbit Clitoral Smooth Muscle Cell Culture in vitro |
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| Liu Bo,Liu Jihong,Wang Tao. Biological Characteristics of Female Rabbit Clitoral Smooth Muscle Cell Culture in vitro[J]. National journal of andrology, 2004, 10(3): 198-201 |
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| Authors: | Liu Bo Liu Jihong Wang Tao |
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| Affiliation: | Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. |
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| Abstract: | Objective: To investigate the method of culturing rabbit clitoral smooth muscle cells(SMC) in vitro and their biological characteristics. Methods: Rabbit clitoral SMCs were cultured in vitro by enzymatically dispersed method, and morphological observation, cell counting and immunohistochemical staining were employed to study their biological characteristics. Results: Rabbit clitoral SMCs were spindle shaped and parallelled along their longitudinal axis, and the attachment and proliferation in vitro were rapid. Conclusion: Rabbit clitoral SMCs cultured in vitro can grow and maintain their steady characteristics when provided with appropriate culture condition. Clitoral SMCs cultured in vitro can serve as a cell material for researches of clitoris. |
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| Keywords: | clitoral corpus cavernosum smooth muscle cell cell culture in vitro rabbit female |
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