胆红素脑病仔鼠脑组织S-100的mRNA及其蛋白变化研究

目的：观察胆红素脑病仔鼠脑组织S-100蛋白（S-100）的mRNA及其蛋白变化．探讨其对胆红素脑病的诊断价值和其分子生物学机制。方法：采用新生7d龄清洁级Wistar大鼠腹腔注射胆红索200mg／kg制备胆红素脑病模型。应用逆转录-聚合酶链反应（RT—PCR）技术动态观察胆红素脑病仔鼠不同时间点脑组织S-100 mRNA表达变化；应用免疫组织化学方法动态观察不同时间点脑组织S-100蛋白表达变化。结果：实验组脑组织S-100 mRNA表达12h明显升高．48h达峰值．至96h与对照组差异无显著性意义：造模后12h海马区脑组织S-100阳性表达明碌升高，48h达峰值，至96h与对照组无显著性差异；皮质区6h明显升高。48h达峰值，至96h与对照组差异无显著性意义；丘脑区6h明显升高，72h下降，至96h与对照组无显著性差异；苍白球区6h明显升高，48h达峰值，72h下降，至96h与对照组比较仍有显著性差异。结论：S-100mRNA的变化同S-100蛋白的变化趋势一致，是判定胆红素脑病的可靠神经生化指标。

S-100 mRNA and protein expression in offspring rats brain tissue with bilirubin encephalopathy

Objective:To observe the dynamic changes of S-100 mRNA and protein expression bilirubin encephalopathy offspring rats. Method: RT-PCR was used to analyze the dynamic changes of S-100 mRNA at 6h, 12h, 24h,48h, 72h, 96h in offspring rats brain tissue with bilirubin encephalopathy. Immunohistochemistry was used for evaluation of S-100 protein expression at 6h, 12h, 24h, 48h, 72h, 96h. Result: The expressions of S-100 mRNA were significantly increased in offspring rats brain tissue with bilirubin encephalopathy within 12h and reached peak value at 48h compared with the control groups and then gradually decreased. The expressions of S-100 protein in hippocampus were significantly increased from 12h to 72h compared with the control group and reached peak value at 48h and then gradually decreased. The expressions of S-100 protein in cerebral cortex were significantly increased from 6h to 72h, and reached peak value at 48h and gradually decreased afterward. The expressions of S-100 protein in thalamic and pallidus were significantly increased from 6h to 72h compared with the control groups, and reached peak value at 48h and then gradually decreased. Conclusion: The changes trends of S-100 mRNA were identical to those of S-100 protein; S-100 may reflect the degree of injury of neurogliocyte. It might be a reliable index to detect bilirubin encephalopathy.