高效液相色谱法测定两性霉素B在家兔体内的血药浓度及药动学

目的：建立血浆中两性霉素B浓度的HPLC测定方法，并用此方法研究两性霉素B在家兔体内的药动力学，方法：血浆经处理后，采用PlatinumC18柱以乙腈-0.005mol.L^-1EDTA-2Na(60:40)为流动相，在紫外405nm处检测。结果：两性霉素B浓度在0．05－12．8ug.ml^-1范围内与色谱峰面积呈良好的线性关系，血浆中药物最低检测浓度为0．005ug.ml^-1，高中低三种浓度的日内及日间RSD％（n=5)分别为（0．34－1．71％）和（0．25－6．78）％，方法回收率和绝对回收率分别为（100．03－105．19）％和（92．18－95．21），药动研究表明，家兔静脉注射单剂量的两性霉素B后其体内血药浓度时间数据符合权重为1／C的三室模型。结论：本方法操作简便，结果准确可靠,很好的满足两性霉素B血药浓度测定及药动学的研究。

Determination ofamphotericin B in rabbits plasma and pharmacokinetics investigation by HPLC

OBJECTIVE：To establish an HPLC method for the determination of amphotericin B (AmB) in rabbits plasma and investigate the pharmacokinetic characteristics of AmB by intravenous administration. METHODS：Platinum C18 column and acetonitrile-EDTA-2Na(60∶40) as mobile phase, with UV detection at 405 nm.RESULTS：A good linear relationship was obtained between the peak area and the concentration of AmB from 0.05～12.8 μg*ml-1, and the limit of detection was 0.005 μg*ml-1. The RSD of intra-day and inter-day variations were 0.34%～1.71% and 0.25%～6.78% respectively. The absolute and relative recoveries were 92.18%～95.21% and 100.03%～105.19% respectively. The concentration-time data of AmB after intravenous administration were fitted by three compartments with weight of 1/C. CONCLUSIONS：A simple, highly sensitive and selective method for the determination of AmB level in plasma was established,which could be used for its pharmacokinetic studies.