凋亡抑制蛋白c-FLIP在带状疱疹患者细胞免疫中作用的研究

目的探讨凋亡抑制蛋白c-FLIP在抗水痘-带状疱疹病毒(VZV)免疫中的作用。方法采用流式细胞术检测30例无恶性肿瘤的带状疱疹患者、17例伴恶性肿瘤的带状疱疹患者和20例正常对照者外周血T细胞和B细胞内c-FLIP蛋白表达阳性率和CD3+T细胞阳性率。结果急性期伴有恶性肿瘤的带状疱疹患者外周血T细胞内c-FLIP表达明显低于无恶性肿瘤和正常对照组(P<0.05,P<0.01),无恶性肿瘤组明显低于正常对照组(P<0.05);恢复期无恶性肿瘤的带状疱疹患者外周血T细胞内c-FLIP表达明显增加,与急性期相比有明显差异(P<0.01),但恢复期的伴恶性肿瘤和急性期相比无明显差异(P>0.05);外周血B细胞内c-FLIP表达在急性期和恢复期的带状疱疹患者中无明显差异(P>0.05)。CD3+T细胞在带状疱疹患者外周血中的阳性率与c-FLIP有相似的表达趋势,两者呈正相关(r分别为0.806和0.534,P均<0.05)。结论凋亡抑制蛋白c-FLIP在急性期带状疱疹患者外周血T细胞内低表达,恢复期则高表达,且与T细胞数量明显正相关,表明其可能参与带状疱疹患者T细胞的增殖,在带状疱疹发生、发展中发挥一定的作用。

Study on the Role of Apoptosis Inhibitory Protein-cellular FLIP in Cellular Immunity in Patients with Herpes Zoster.

Objective To investigate the role of apoptosis inhibitory protein-cellular FLIP in cellular immunity against varicella-zoster virus in herpes zoster (HZ).Methods Flow cytometry was used for simultaneous detection of intracellular c-FILP protein in T and B lymphocytes and positive rates of CD3+T lymphocytes in peripheral blood in 30 HZ patients without tumor, 17 HZ patients with tumor and 20 normal controls. Results Significant decrease of c-FLIP expression in T lymphocytes was found in acute HZ patients with tumor compared to that without tumor and normal controls (P<0.05 and P<0.01, respectively), and it decreased in acute HZ patients without tumor compared to normal controls (P<0.05). Significant increase of c-FLIP expression in T lymphocytes was found in convalescent HZ patients without tumor compared to that in the acute stage (P<0.01), but its expression in convalescent HZ patients with tumor showed no significance when compared to that in the acute stage (P>0.05). There was no significant difference of c-FLIP expression in B lymphocytes between in acute and convalescent stage of HZ patients (P>0.05). Positive rates of CD3+T lymphocytes had the similar approach as c-FLIP and the positive relationship with c-FLIP expression(r=0.806 and 0.534,P<0.05). Conclusion Low and high expressions of c-FLIP were found in T lymphocytes in peripheral blood in acute and convalescent HZ patients respectively and had a positive correlation with CD3+T lymphocytes, suggesting that c-FLIP might take part in the proliferation of T lymphocytes and play a crucial role in the pathogenesis and progression of HZ.