RP-HPLC测定六味地黄方中苷类成分的含量

目的 :建立RP HPLC测定六味地黄方中苷类成分含量的方法。方法 :选用C₁₈色谱柱 ,甲醇-水 (33∶67)为流动相 ,检测波长 236nm ,柱温 30℃下进行含量测定。结果 :六味地黄方水煎液中莫诺苷、马钱素、芍药苷进样浓度分别在 7.4～60 ,7.7～62 ,8.5～68mg·L^-1 与其色谱峰面积呈良好线性关系 ,加样回收率分别为 98.8% ,98.3 % ,99.6 %。结论 :本方法简便、快速、准确 ,可作为六味地黄制剂中苷类部位的质量检测方法。

Determination of Glycosides in Traditional Chinese Medicine Liu-wei Di-huang by RP-HPLC

OBJECTIVE: To establish a RP-HPLC method for determination of glycosides in Traditional Chinese Medicine Liu-wei Di-huang. METHOD: The samples were analyzed on an ODS column at 30 degrees C, with mobile phase of methanol/water (33:67) at flow rate 1.0 mL.min and detection at wavelength of 236 nm. RESULT: Three major components reached base-line separation and were identified to be mononiside, loganin, paeoniflorin. Respectively for the three components, linear correlations were found between peak areas and concentrations in the ranges of 7.4-60, 7.7-62 mg.L-1 and 8.5-68 mg.L-1, and the recoveries were 98.8%, 98.3%, 99.6%. CONCLUSION: The established method is proved to be suitable for simultaneous quantification of three major glycosidic components in Liuwei Dihuang decoction and can be used for evaluation of the quality of Liuwei Dihuang preparations.