正相液相-气相-质谱联用技术分离分析金银花挥发油化学成分

采用离线正相色谱-气相色谱（NPLC—GC）联用技术对山东郑城镇金银花挥发油进行了良好的分离，并采用气相色谱／四极杆质谱（GC／qMS）和气相色谱/正交加速飞行时间质谱（GC／oaTOFMS）对其进行了联合定性。基于NPLC—GC强大的分离功能，结合GC／qMS的谱库检索功能以及GC／oa TOFMS对质谱碎片离子精确的质量测定功能，成功地定性了其中的近两百个化合物。对复杂体系的良好分离是实现化合物准确定性的前提，而采用多种色谱联用定性较采用单一的联用技术具有更高的可靠性。

Separation and Analysis of Volatile Oil from Lonicera japonica thunb Using Normal Phase Liquid Chromatography-Gas Chromatography Hyphenated with Mass Spectrometry

Offline normal phase liquid chromatography-gas chromatography （NPLC-GC） was applied to separate the volatile oil from lonicera japonica thumb. The qualitative analysis was carried out on gas chromatography / quadrupole mass spectrometry （GC/qMS） and gas chromatography/orthogonal acceleration time-of-flight mass spectrometry （GC/ oaTOFMS）. The efficient separation of NPLC-GC and the powerful qualitative capability of GC/qMS and GC/oaTOFMS have resulted in the identification of some two hundred volatile compounds. Compared with the results obtained from using GC/qMS alone, the qualitative results obtained from using multiple hyphenated techniques are more reliable.