猫眼草水提物对小鼠Lewis肺癌的抑制作用及机制探讨

目的:探讨中药猫眼草对小鼠Lewis肺癌的生长抑制、免疫调节、血液系统、细胞周期的影响,并对其抗癌作用机制进行探讨。方法:选择C₅₇BL/6小鼠移植性Lewis肺癌为实验模型进行试验,观察猫眼草对肺癌的抑制、免疫调节以及对小鼠血细胞的影响,同时采用流式细胞仪检测瘤组织的细胞周期、凋亡率,应用TUNEL法观察猫眼草对肺癌细胞凋亡的影响。结果:①猫眼草7.5,15,30,60 g.kg^-1组抑瘤率分别为0.61%,16.93%,32.81%和58.26%,与模型组相比30,60 g.kg^-1组有显著性差异(P<0.05)。②猫眼草各组对荷瘤小鼠胸腺指数无明显影响,60 g.kg^-1组可显著降低荷瘤小鼠脾指数(P<0.05)。③猫眼草各组对荷瘤小鼠血液系统无影响。④流式细胞仪检测猫眼草30,60 g.kg^-1组可使Lewis肺癌组织肿瘤细胞S期细胞比例增高,凋亡率分别为(16.43±18.69)%,(24.37±15.48)%,与模型组相比有显著性差异(P<0.05)。⑤TUNEL法测定猫眼草30,60 g.kg^-1组的凋亡指数(AI)分别为(4.00±7.15)%,(5.93±5.96)%;60 g.kg^-1与模型组相比有显著性差异,有明显诱导细胞凋亡的作用(P<0.01)。结论:猫眼草可抑制Lewis肺癌生长,对小鼠血液系统无明显毒副作用,其抗肿瘤作用与诱导细胞凋亡有关。

Experimental study on the theraputic effect of crescent euphorbia on lewis lung cancer in mice

OBJECTIVE: To observe the inhibitory effects of crescent euphorbia on tumor growth, immunoregulation, the side effect on the mice blood system, cell cycle and to investigate its effect on apoptosis of tumor cells. METHOD: Crescent euphorbia was administered gastrally to C57BL/6J mouse implanted with Lewis lung cancer for 12 days. Inhibition on tumor growth, immunoregulation,the side effect on the mice blood system and cell cycle were observed and its effect on apoptosis of lung cancer cells were investigated. RESULT: (1) Inhibitory rates of crescent euphorbia at doses of 7.5, 15, 30 g x kg(-1) and 60 g x kg(-1) were 0.61%, 16.93%, 32.81% and 58.26% respectively, significant differenel with those of controls at doses of 30 g x kg(-1) and 60 g x kg(-1)(P < 0.05). (2) Although no effect on thymus index in normal mouse at all dose of crescent euphorbia was found, spleen index in tumor-bear mice was reduced significantly at dose of 60 g x kg(-1)(P < 0.05). (3) No side effect on the miceblood system was found. (4) The percentage of S phase of cell cycle was increased in the group of crescent euphorbia at dose of 30 g x kg(-1), 60 g x kg(-1) and the apoptotic rate was (16.43 +/- 18.69)% and (24.37 +/- 15.48)% by flow eytometry (P < 0.05). The apoptotic index (AI)of these two groups were (4.00 +/- 7.50)% and (5.93 +/- 5.96)% by TUNEL, however apoptosis was found in the group at doses of 60 g kg(-1) (P < 0.01). CONCLUSION: Crescent euphorbia can inhibit the growth of Lewis lung cancer in mice, no side effection for the mice blood system and the effect might be associated with induction of apoptosis on cancer cells.