肺腺癌患者术后血清蛋白质组构型动态随访研究

目的用表面增强激光解析离子化飞行时间质谱(surface-enhanced laser desorption ionization time of flightmass spectrometry,SELDI-TOF-MS)技术建立肺腺癌患者血清蛋白质组构型,并筛选出相对特异标记物,随访分析其手术后的动态变化及意义。方法肺腺癌患者71例,均经手术病理证实;正常志愿者71例,按照性别、年龄、吸烟史与71例肺腺癌患者配对。用弱阳离子交换芯片(WCX2)检测各血清标本,筛选肺腺癌相对特异性蛋白质峰。随访分析71例肺腺癌患者手术后3个月、6个月和9个月血清蛋白质组构型变化。结果与正常志愿者比较发现肺腺癌患者有5个显著高表达的潜在标记物,相对分子量分别为4 047.79,4 203.99,4 959.81,5 329.30和7 760.12 Da;肺腺癌患者手术后血清蛋白质组构型变化的个体差异较大,并与患者病理分期密切相关。结论SELDI-TOF-MS蛋白质质谱技术是一种快速、简便易行且高通量的分析方法,能直接筛选出肺腺癌患者血清中相对特异的潜在标记物,且可能具有较好的临床应用前景。

Follow-up Analysis of Postoperative Serum Proteomic Patterns in Patients of Lung Adenocarcinoma

Objective To select relatively specific biomarkers in serum from lung adenocarcinoma patients using surface-enhanced laser desorption ionization time of flight mass spectrometry(SELDI-TOF-MS) Protein Chip technology,and study the follow-up results of postoperative serum proteomic patterns.Methods Serum samples from 71 lung adenocarcinoma patients,71 healthy volunteers with matched gender,age and history of smoking were analyzed by using weak cation exchange 2(WCX2) Protein Chip to select potentially biomarkers.Seventy-one patients were followed-up till 9 months after surgery.Compare the serum proteomic patterns 3,6 and 9 months after surgery.(Results)Five highly expressed potential biomarkers were identified with the relative molecular weights of(4 047.79,) 4 203.99,4 959.81,5 329.30 and 7 760.12 Da.The postoperative serum proteomic patterns changed among individuals,and correlated with patients' clinical stage.Conclusions SELDI-TOF-MS Protein Chip technology is a quick,easy,convenient,and high-throughout analyzing method capable of selecting relatively specific,potential biomarkers from the serum of lung adenocarcinoma patients and may have attractive clinical value.