| UHPLC-Q-TOF／MS技术应用于中药旱莲草化学成分研究 |
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| 引用本文： | 夏爱军,李玲,董昕,娄子洋,梁园,张琪.UHPLC-Q-TOF／MS技术应用于中药旱莲草化学成分研究[J].解放军药学学报,2012,28(5):404-407. |
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| 作者姓名： | 夏爱军 李玲 董昕 娄子洋 梁园 张琪 |
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| 作者单位： | 1. 解放军第303医院药剂科,广西南宁,530021
2. 第二军医大学药学院,上海,200433 |
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| 基金项目： | 广西自然科学基金项目,No.2010GXNSFA 013270 |
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| 摘 要： | 目的通过超高效液相色谱-四极杆飞行时间串联质谱（UHPLC-Q-TOF/MS）对中药旱莲草的化学成分进行系统、全面研究。方法采用Agilent SB-C18（2.1 mm×100 mm,1.8μm）柱,流动相为乙腈-甲酸溶液系统梯度洗脱,质谱采用ESI正离子模式,分别对桂林阳朔、桂林荔蒲、湖南邵阳、南宁和湖北襄樊五个产地的旱莲草药材进行分析。结果该方法共鉴别出旱莲草中29个化学成分。结论本实验为建立旱莲草药材的质量控制标准提供了参考依据。
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| 关 键 词： | 旱莲草 UHPLC 四极杆飞行时间串联质谱 中药质量控制 |
Studies on Chemical Constituents of Eclipta Prostrata by UHPLC-Q-TOF/MS |
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| XIA Ai-jun
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LI Ling
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DONG Xin
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LOU Zi-yang
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LIANG Yuan
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ZHANG Qi.Studies on Chemical Constituents of Eclipta Prostrata by UHPLC-Q-TOF/MS[J].Pharmaceutical Journal of Chinese People's Liberation Army,2012,28(5):404-407. |
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| Authors: | XIA Ai-jun LI Ling DONG Xin LOU Zi-yang LIANG Yuan ZHANG Qi |
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| Institution: | ② ①Department of Pharmacy,No.303 Hospital of PLA,Nanning 530021,China; ②College of Pharmacy,Second Military Medical University,Shanghai 200433,China |
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| Abstract: | Objective To analyze chemical constituents of Eclipta Prostrata using UHPLC-Q-TOF/MS.Methods Agilent SB-C18 column(2.1 mm×100 mm,1.8 μm) was used for separation and identification of chemical components in Eclipta Prostrata with a mobile phase of acetonitrile-water system in gradient elution.Q-TOF/MS was applied for qualitative analysis under a positive ion mode.Five origins of Eclipta Prostrata were analyzed.Results Under the UHPLC/MS condition,29 compounds were identified in Eclipta Prostrate.Conclusion This method provides reference for establishing quality control standards of Eclipta Prostrata. |
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| Keywords: | Eclipta Prostrata UHPLC Q-TOF/MS quality control of TCM |
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